Screening of Rice Endophytic Biofertilizers with Fungicide Tolerance and Plant Growth-Promoting Characteristics

The application of pesticides is unavoidable in conventional agricultural practice. To develop effective biofertilizers, bacterial strains with both pesticide tolerance and plant growth-promoting (PGP) traits were isolated for further testing. Seedlings of rice (Oryza sativa) were planted in soil wi...

Full description

Saved in:
Bibliographic Details
Published inSustainability Vol. 11; no. 4; p. 1133
Main Authors Shen, Fo-Ting, Yen, Jui-Hung, Liao, Chien-Sen, Chen, Wen-Ching, Chao, Yi-Ting
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 21.02.2019
Subjects
Acetic acid
Agricultural practices
Agrochemicals
Aquatic plants
Bacillus aryabhattai
Bacteria
Endophytes
Fertilizers
Fungicides
Indoleacetic acid
Metalaxyl
Nitrogen
Nitrogen fixation
Nitrogenation
Pesticides
Phosphorus
Plant growth
Rice
Roots
rRNA 16S
Seedlings
Seeds
Soils
Strains (organisms)
Tryptophan
Taiwan
Online AccessGet full text

Cover

More Information
Summary:The application of pesticides is unavoidable in conventional agricultural practice. To develop effective biofertilizers, bacterial strains with both pesticide tolerance and plant growth-promoting (PGP) traits were isolated for further testing. Seedlings of rice (Oryza sativa) were planted in soil with 1, 5, or 10 times the recommended rates of the fungicides etridiazole, metalaxyl, and tricyclazole. Endophytic bacteria were isolated from roots of rice seedlings. The bacterial 16S rDNA sequences and related PGP characteristics including potential nitrogen fixation, phosphorus-solubilizing and indole acetic acid (IAA) production ability were further examined. In all, 17 different strains were obtained from rice seedling roots; five strains with both nitrogen fixation potential and IAA production ability included Rhizobium larrymoorei E2, Bacillus aryabhattai E7, Bacillus aryabhattai MN1, Pseudomonas granadensis T6, and Bacillus fortis T9. Except for T9, all strains could tolerate two or more fungicides. We inoculated rice roots with the endophytic bacteria and all conferred rice growth-promoting ability. Bacillus aryabhattai MN1 was further tested and showed high tryptophan dose-dependent IAA production ability, tolerance towards etridiazole and metalaxyl application and should be considered a potential bacterial biofertilizer.
ISSN:2071-1050
2071-1050
DOI:10.3390/su11041133