Chitin-glucan composite sponge hemostat with rapid shape-memory from Pleurotus eryngii for puncture wound

• Published in: Carbohydrate polymers Vol. 291; p. 119553
• Main Authors: Sun, Caixia, Yue, Pengpeng, Chen, Runlan, Wu, Shuangquan, Ye, Qifa, Weng, Yunxiang, Liu, Haiqing, Fang, Yan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2022
• Subjects: Animals; Chitin; Chitin - pharmacology; Chitin - therapeutic use; Composite; Glucans; Hemorrhage - drug therapy; Hemostatic sponge; Hemostatics - chemistry; Hemostatics - pharmacology; Hemostatics - therapeutic use; Pleurotus; Punctures; Rats; Water-triggered shape-memory; Hemostatic sponge; Chitin; Composite; Glucans; Water-triggered shape-memory
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2022.119553

Efficient hemostasis is a great challenge for treating the inaccessible hemorrhage wounds. A novel shape-memory chitin-glucan hemostatic sponge (ATC-Sponge) is constructed via sequentially in-situ removal of protein and glucan from Pleurotus eryngii fruiting body, TEMPO oxidation and Ca2+ crosslinking. The sponge displays interconnected microporous structure with high water absorption and robust mechanical properties. The sponge at dry state shows rapid blood-triggered shape-memory, allowing easy insertion into the puncture wound in a compressed fixed-shape and the subsequent quick volume expansion to conform wound shape to stop bleeding. Compared with standard medical gauze and gelatin sponge, ATC-Sponge demonstrates superior hemostatic performance in the rat femoral artery and non-compressive liver puncture injury models. Additionally, ATC-Sponge can effectively accelerate wound healing. This multi-functional shape-memory ATC-Sponge shows high potential in controlling the bleeding of inaccessible traumas.