Biosynthesis of monensins A and B: the role of isoleucine

Isoleucine added to the cultivation medium of Streptomyces cinnamonensis C-100-5 induced a relative increase of the production of monensin B at the expense of monensin A. U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of 1-13C-2-methylbutyrate into monensins...

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Bibliographic Details
Published inFolia microbiologica Vol. 31; no. 1; p. 8
Main Authors Pospísil, S, Sedmera, P, Krumphanzl, V, Vanĕk, Z
Format Journal Article
LanguageEnglish
Published United States 1986
Subjects
Aminobutyrates - pharmacology
Butyrates - metabolism
Furans - biosynthesis
Isoleucine - metabolism
Isoleucine - pharmacology
Monensin - analogs & derivatives
Monensin - biosynthesis
Mutation
Norleucine - pharmacology
Streptomyces - genetics
Streptomyces - metabolism
Valine - biosynthesis
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Summary:Isoleucine added to the cultivation medium of Streptomyces cinnamonensis C-100-5 induced a relative increase of the production of monensin B at the expense of monensin A. U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of 1-13C-2-methylbutyrate into monensins A and B showed the label to be evenly incorporated in both products at carbon atoms originating from C(1) of propionate. In regulatory mutants insensitive to 2-amino-3-chlorobutyrate isoleucine influenced the production of monensins only slightly but strains resistant to 2-aminobutyrate and norleucine decreased their total production by 2-12% in the presence of isoleucine which was associated with a decrease of monensin A content by 14-52%. The inhibitory effect of isoleucine on the biosynthesis of valine, a specific precursor of the butyrate unit of monensin A, is discussed.
ISSN:0015-5632
DOI:10.1007/BF02928674