Bacterial endophytes of Trans-Ili Alatau region's plants as promising components of a microbial preparation for agricultural use

• Published in: Acta biochimica Polonica Vol. 63; no. 2; p. 321
• Main Authors: Mukasheva, Togzhan, Berzhanova, Ramza, Ignatova, Lyudmila, Omirbekova, Anel, Brazhnikova, Yelena, Sydykbekova, Raikhan, Shigaeva, Maya
• Format: Journal Article
• Language: English
• Published: Poland 01.01.2016
• Subjects: Agriculture; Crops, Agricultural - growth & development; Crops, Agricultural - microbiology; Endophytes - isolation & purification; Endophytes - metabolism; Glycine max - growth & development; Glycine max - microbiology; Hordeum - growth & development; Hordeum - microbiology; Indoleacetic Acids - metabolism; Kazakhstan; Plant Growth Regulators - biosynthesis; Plant Roots - growth & development; Plant Roots - microbiology; Streptomyces - isolation & purification; Streptomyces - metabolism
• ISSN: 0001-527X; 1734-154X
• DOI: 10.18388/abp.2015_1157

In this study, 382 isolates of bacterial endophytes from tissues of plants grown in the foothills and piedmont plains of the Trans-Ili Alatau were isolated. It was found that certain strains actively produce indole-3-acetic acid (IAA) in a medium containing l-tryptophan. Among the strains studied, 26 strains (66%) showed a positive response to production of IAA. Bacteria synthesized IAA in the range of 18.6 ± 1.1 to 82.4 ± 2.3 µg/ml. IAA was synthesized more actively by Streptosporangium sp. KK1 (44.1 µg/ml), Rhodococcus sp. KK 2 (42.5 µg/ml), Streptomyces tendae KK3 (44.9 µg/ml) strains. The most active auxin's producer is a Jeotgalicoccus halotolerans BAK1 strain, whose total level of IAA production reached 82.4 µg/ml. Such strains as Streptomyces griseorubiginosis KK4, Streptomyces sp. KK5 and Jeotgalicoccus halotolerans BAK1 were found to have a significant stimulating effect which was reflected in the increase of the length of the roots of soybean and barley. As a result, 8 promising strains with fungicidal, growth-stimulating, phosphorus dissolving and enzymatic activities were selected for the further development of highly microbiological preparations for crop research.