Reduction of Shoot Hyperhydricity in Micropropagated Potato Plants via Antisense Inhibition of a chCu/ZnSOD Gene

• Published in: Applied biological chemistry Vol. 52; no. 4; pp. 397 - 400
• Main Authors: Lee, Y.H., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Kim, H.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Kim, M.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Kim, Y.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Joung, H., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Jeon, J.H., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.08.2009; Springer Nature B.V; 한국응용생명화학회
• Subjects: Antisense DNA; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; ETHYLENE; ETILENO; hyperhydricity; MICROPROPAGACION; MICROPROPAGATION; PAPA; POMME DE TERRE; POTATOES; Short Communications; SUPEROXIDE DISMUTASE; SUPEROXIDO DISMUTASA; SUPEROXYDE DISMUTASE; Transgenic plants; 농학; hyperhydricity; micropropagation; potato; superoxide dismutase; H; ethylene; O
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.3839/jksabc.2009.070

The occurrence of hyperhydricity in transgenic potato plants, which carry a chloroplastic Cu/ZnSOD gene of the lily in either a sense or antisense orientation, was investigated during in vitro culturing. Hyperhydric symptoms included curled leaves with a succulent appearance that were mainly observed in Cu/ZnSOD sense plants. By contrast, antisense plants showed a low frequency of hyperhydricity and a high rate of ex vitro survival. Therefore, a knockdown approach for a chCu/ZnSOD gene could be a practical method to reduce hyperhydricity in plant micropropagation.