Steroidobacter gossypii sp. nov., isolated from cotton field soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 71; no. 8
• Main Authors: Huang, Rui-Rui, Ge, Xian-Feng, Chen, Xin-Kai, Yang, Shen-Rong, Zhen, Cheng, Wen, Zhi-Qiang, Li, Ya-Nan, Liu, Wen-Zheng
• Format: Journal Article
• Language: English
• Published: England 01.08.2021
• Subjects: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Gammaproteobacteria - classification; Gammaproteobacteria - isolation & purification; Gossypium - microbiology; Nucleic Acid Hybridization; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone - chemistry; China; Steroidobacter; cotton field; soil
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004935

A Gram-negative bacterium, designated S1-65 , was isolated from soil samples collected from a cotton field located in the Xinjiang region of PR China. Results of 16S rRNA gene sequence analysis revealed that strain S1-65 was affiliated to the genus with its closest phylogenetic relatives being ' ' 35Y (98.4 %), ' ' SA29-B (98.3 %) and KA5-B (98.3 %). 16S rRNA-directed phylogenetic analysis showed that strain S1-65 formed a unique phylogenetic subclade next to ' ' SA29-B and KA5-B , suggesting that strain S1-65 should be identified as a member of the genus . Further, substantial differences between the genotypic properties of strain S1-65 and the members of the genus , including average nucleotide identity and digital DNA-DNA hybridization, resolved the taxonomic position of strain S1-65 and suggested its positioning as representing a novel species of the genus . The DNA G+C content of strain S1-65 was 62.5 mol%, based on its draft genome sequence. The predominant respiratory quinone was ubiquinone-8. The main fatty acids were identified as summed feature 3 (C ω6 /C ω7 ), C and iso-C . In addition, its polar lipid profile was composed of aminophospholipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Here, we propose a novel species of the genus : sp. nov. with the type strain S1-65 (=JCM 34287 =CGMCC 1.18736 ).