Adrenaline profiling of lipases and esterases with 1,2-diol and carbohydrate acetates

• Published in: Tetrahedron Vol. 60; no. 3; pp. 703 - 710
• Main Authors: Wahler, Denis, Boujard, Olivier, Lefèvre, Fabrice, Reymond, Jean-Louis
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 12.01.2004
• Subjects: Adrenaline; Biocatalysis; Esterases; Adrenaline; Esterases; Biocatalysis
• ISSN: 0040-4020; 1464-5416
• DOI: 10.1016/j.tet.2003.11.059

The adrenaline test for enzymes is a general back-titration procedure to detect 1,2-diols, 1,2-aminoalcohols and α-hydroxyketones reaction products of enzyme catalysis by colorimetry. The method was used to profile a series of esterases and lipases for their esterolytic activity on a series of carbohydrate and polyol acetates. Substrates were prepared by peracetylation and used for parallel microtiter-plate analysis of enzyme activities. This method can be used to achieve a rapid and automated characterization of a set of enzymes during HTS screening. The adrenaline test for enzymes is a general back-titration procedure to detect 1,2-diols, 1,2-aminoalcohols and α-hydroxyketones reaction products of enzyme catalysis by colorimetry. The method was used to profile a series of esterases and lipases for their esterolytic activity on a series of carbohydrate and polyol acetates. Substrates were prepared by peracetylation and used for parallel microtiter-plate analysis of enzyme activities. This method can be used to achieve a rapid and automated characterization of a set of enzymes during HTS screening.