Pseudomonas kunmingensis sp. nov., an exopolysaccharide-producing bacterium isolated from a phosphate mine

• Published in: International journal of systematic and evolutionary microbiology Vol. 64; no. Pt 2; pp. 559 - 564
• Main Authors: Xie, Fuhong, Ma, Huan, Quan, Shujing, Liu, Dehai, Chen, Guocan, Chao, Yapeng, Qian, Shijun
• Format: Journal Article
• Language: English
• Published: England 01.02.2014
• Subjects: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Mining; Molecular Sequence Data; Nucleic Acid Hybridization; Phosphates; Phylogeny; Pseudomonas - classification; Pseudomonas - genetics; Pseudomonas - isolation & purification; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone - chemistry
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.055632-0

A Gram-stain-negative, rod-shaped, exopolysaccharide-producing, strictly aerobic bacterium with a single polar flagellum, designated strain HL22-2(T), was isolated from a phosphate mine situated in a suburb of Kunmming in Yunnan province in south-western China. The taxonomic status of this strain was evaluated by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HL22-2(T) was related to members of the genus Pseudomonas. 16S rRNA gene sequence similarities between strain HL22-2(T) and Pseudomonas xanthomarina KMM 1447(T), Pseudomonas alcaliphila AL15-21(T) and Pseudomonas stutzeri ATCC 17588(T) were 98.9, 98.10% and 98.06%, respectively. The major cellular fatty acids were C(18 : 1)ω7c, C(16 : 0) and summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c). The DNA G+C content was 60.3 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness values, strain HL22-2(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas kunmingensis sp. nov. is proposed. The type strain is HL22-2(T) ( = CGMCC 1.12273(T) = DSM 25974(T)).