Intermittent fasting, high-intensity interval training, or a combination of both have beneficial effects in obese mice with nonalcoholic fatty liver disease

• Published in: The Journal of nutritional biochemistry Vol. 104; p. 108997
• Main Authors: de Castro-de-Paiva, Patrícia, Marinho, Thatiany de Souza, Mandarim-de-Lacerda, Carlos Alberto, Aguila, Marcia Barbosa
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2022
• Subjects: Adiponectin - metabolism; Animals; Diet, High-Fat - adverse effects; Fasting; Female; high-intensity interval training; High-Intensity Interval Training - methods; Interleukin-6 - metabolism; intermittent fasting; Leptin - metabolism; Liver - metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Obese; molecular biology; Non-alcoholic Fatty Liver Disease - metabolism; Non-alcoholic Fatty Liver Disease - therapy; Nonalcoholic fatty liver disease; Obesity - complications; Obesity - metabolism; Obesity - therapy; Plasminogen Activator Inhibitor 1 - metabolism; pro-inflammatory markers; molecular biology; NAFLD; C; Nrf; pro-inflammatory markers; Srebf; HIIT; TNF; LPS; AUC; BW; LDL; Nonalcoholic fatty liver disease; Tlr; IF; high-intensity interval training; Pgc1; CD; IL; FGF; FNDC; Cpt1; TC; intermittent fasting; QUICKi; Ppar; PAI; FIRi; FA; MCP; TAG; OGTT; HF; MRC
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2022.108997

Intermittent fasting (IF) and high-intensity interval training (HIIT) are procedures that might mitigate the effects of nonalcoholic fatty liver disease. Two groups of 3-month-old C57BL/6 male mice were fed for 16 weeks with a control (C) or high-fat (HF) diet. In the last 4 weeks of the study, IF, HIIT, and IF/HIIT were implemented. Obese HF animals showed liver fat accumulation with macro-, and micro-vesicular steatosis and inflammatory infiltrate. IF and HIIT successfully reduced liver steatosis in the HF-derived groups. IF, HIIT, and IF/HIIT were beneficial in improving glucose metabolism in both C-derived and HF-derived groups. High levels observed in plasmatic and liver levels of total cholesterol and triacylglycerol in the HF group compared to the C group were mitigated by IF, HIIT, and IF/HIIT. IF decreased adiponectin and increased leptin and insulin in the HF group. HIIT improved adiponectin and leptin. IF chances liver gene expressions: increased interleukin-6 (IL-6) in the C IF group, reduced IL-6, and PAI-1 in the HF group. IF/HIIT reduced IL-6, MCP-1, and PAI-1. IF and HIIT enhanced hepatic beta-oxidation. However, lipogenesis was reduced by IF and HIIT in the HF-derived groups. In conclusion, IF and HIIT benefit weight loss, hormones, glucose tolerance/insulin resistance, liver steatosis/inflammation, fatty acid oxidation, and lipogenesis. Furthermore, the IF groups showed beneficial effects more often and intensely than HIIT ones. The IF/HIIT combination was slightly more efficient than IF, indicating that IF is the primary intervening factor benefiting the obese mouse liver.