A comparison of in vitro and in vivo estimates of the viability of Taenia pisiformis eggs aged under controlled conditions, and their ability to immunise against a challenge infection

• Published in: International journal for parasitology Vol. 7; no. 1; pp. 15 - 20
• Main Authors: Coman, B.J., Rickard, M.D.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.1977
• Subjects: ageing process; Animals; Female; hatching; Liver - parasitology; Ovum - immunology; Ovum - physiology; Preservation, Biological; Rabbits; senescent eggs; sterile immunity; Taenia - immunology; Taenia - physiology; Taenia pisiformis; Taeniasis - immunology; Taeniasis - parasitology; taeniid egg; viability; taeniid egg; Taenia pisiformis; sterile immunity; ageing process; senescent eggs; hatching; viability
• ISSN: 0020-7519; 1879-0135
• DOI: 10.1016/0020-7519(77)90019-4

Storing the eggs of T. pisiformis for periods of 0 to 9 days at a temperature of 36–38°C and relative humidity of 87–93 % revealed at least 4 stages in the ageing process. Firstly, a stage in which eggs hatched and activated in vitro and were also fully infective for rabbits. Secondly, a stage in which eggs hatched and activated in vitro but underwent only partial development in rabbits. Thirdly, a stage in which eggs hatched and activated in vitro, and presumably therefore in vivo, but no evidence of infection could be found when they were fed to rabbits. Fourthly, a stage in which eggs either did not hatch, or if they did hatch, the oncosphere was rapidly digested. In vitro techniques for assessing viability of T. pisiformis eggs were not a reliable guide to their infectivity for rabbits. ‘Senescent’ eggs, that is eggs which produced evidence of early infection in rabbits but did not complete their development to cysticerci, failed to produce immunity to challenge infection with T. pisiformis eggs.