The mechanism differences between sulfadiazine degradation and antibiotic resistant bacteria inactivation by iron-based graphitic biochar and peroxydisulfate system

• Published in: Journal of hazardous materials Vol. 475; p. 134907
• Main Authors: Ma, Yanbing, Xu, Shengjun, Huang, Yan, Du, Jinge, Wang, Jingzhen, Gao, Boqiang, Song, Jia, Ma, Shuanglong, Jia, Hanzhong, Zhan, Sihui
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2024
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotic resistant bacteria; Bacteria - drug effects; Bacteria - genetics; Bacteria - metabolism; Charcoal - chemistry; Conjugative transfer; Drug Resistance, Bacterial - genetics; Drug Resistance, Microbial - genetics; Graphite - chemistry; Graphitic structure; Iron - chemistry; Iron - metabolism; Iron-based biochar; Peroxides - chemistry; Sulfadiazine - chemistry; Sulfadiazine degradation; Sulfates - chemistry; Sulfates - metabolism; Water Pollutants, Chemical - chemistry; Water Purification - methods; Conjugative transfer; Iron-based biochar; Sulfadiazine degradation; Graphitic structure; Antibiotic resistant bacteria
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.134907

In this study, the activation of peroxydisulfate (PS) by K2FeO4-activation biochar (KFeB) and acid-picking K2FeO4-activation biochar (AKFeB) was investigated to reveal the mechanism differences between iron site and graphitic structure in sulfadiazine (SDZ) degradation and ARB inactivation, respectively. KFeB/PS and AKFeB/PS systems had similar degradation property towards SDZ, but only KFeB/PS system showed excellent bactericidal property. The mechanism study demonstrated that dissolved SDZ was degraded through electron transfer pathway mediated by graphitic structure, while suspended ARB was inactivated through free radicals generated by iron-activated PS, accompanied by excellent removal on antibiotic resistance genes (ARGs). The significant decrease in conjugative transfer frequency indicated the reduced horizontal gene transfer risk of ARGs after treatment with KFeB/PS system. Transcriptome data suggested that membrane protein channel disruption and adenosine triphosphate synthesis inhibition were key reasons for conjugative transfer frequency reduction. Continuous flow reactor of KFeB/PS system can efficiently remove antibiotics and ARB, implying the potential application in practical wastewater purification. In conclusion, this study provides novel insights for classified and collaborative control of antibiotics and ARB by carbon-based catalysts driven persulfate advanced oxidation technology. [Display omitted] •The elimination mechanism difference between antibiotic and ARB was revealed.•The graphitic structure promotes electron transfer from SDZ to PS.•Generated free radicals by iron-activated PS were crucial for disinfection.•The horizontal transfer risk of ARGs can be obviously reduced.