Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 8; pp. 3041 - 3049
• Main Authors: Shkoporov, Andrei N., Chaplin, Andrei V., Shcherbakova, Victoria A., Suzina, Natalia E., Kafarskaia, Lyudmila I., Bozhenko, Vladimir K., Efimov, Boris A.
• Format: Journal Article
• Language: English
• Published: England 01.08.2016
• Subjects: Adult; Bacterial Typing Techniques; Base Composition; Child, Preschool; Clostridiales - classification; Clostridiales - genetics; Clostridiales - isolation & purification; DNA, Bacterial - genetics; Fatty Acids - chemistry; Feces - microbiology; Gram-Positive Bacteria - genetics; Humans; Lactic Acid - biosynthesis; Male; Phylogeny; RNA, Ribosomal, 16S - genetics; Russia; Sequence Analysis, DNA
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.001143

Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1ω9, C18 : 1ω9 aldehyde, C16 : 0 and C16 : 1ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4-56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae, for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T).