Biotransformation of hydroquinone to arbutin in plant in vitro cultures - preliminary results

• Published in: Acta physiologiae plantarum Vol. 27; no. 1; pp. 79 - 87
• Main Authors: Skrzypczak-Pietraszek, E, Szewczyk, A, Piekoszewska, A, Ekiert, H.,Jagiellonian University, Krakow (Poland). Dept. of Pharmaceutical Botany
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer Nature B.V 01.01.2005
• Subjects: ACTIVIDAD CATALITICA; ACTIVITE CATALYTIQUE; Biotransformation; CATALYTIC ACTIVITY; CHEMICAL REACTIONS; CULTIVO IN VITRO; CULTURE IN VITRO; GLUCOSIDE; GLUCOSIDES; GLUCOSIDOS; High-performance liquid chromatography; http://www.fao.org/aos/agrovoc#c_1382; http://www.fao.org/aos/agrovoc#c_24032; http://www.fao.org/aos/agrovoc#c_24135; http://www.fao.org/aos/agrovoc#c_25191; http://www.fao.org/aos/agrovoc#c_4769; http://www.fao.org/aos/agrovoc#c_4788; http://www.fao.org/aos/agrovoc#c_5993; Hydroquinone; IN VITRO CULTURE; Liquid chromatography; METABOLISM; METABOLISME; METABOLISMO; METHODE; METHODS; METODOS; PLANTE; PLANTS; REACCIONES QUIMICAS; REACTION CHIMIQUE; Ruta graveolens
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-005-0039-x

Cells from Echinacea purpurea (L.) Moench. (Asteraceae), Exacum affine Balf. f. (Gentianaceae), Melittis melissophyllum L. (Lamiaceae), Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. (Rutaceae) agitating cultures perform a biotransformation reaction on exogenously supplied hydroquinone into its beta-D-glucoside - arbutin, product with valuable medicinal and cosmetic properties. The maximum content of arbutin (determined by HPLC) in the biomass from investigated cultures is 4.01, 3.44, 1.79, 2.48 and 5.07 g/100 g d.w., respectively. Nothing but Ammi majus L. (Apiaceae) cultures contain trace amounts of the product. Arbutin is accumulated in cells, it is occasionally found in media only in vestigial amounts. In most of the investigated cultures the efficiency of the biotransformation process is about 60 percent