Dyella telluris sp. nov. and Dyella acidiphila sp. nov., isolated from forest soil of Dinghushan Biosphere Reserve, China

• Published in: International journal of systematic and evolutionary microbiology Vol. 71; no. 9
• Main Authors: Huang, Tao, Fu, Jia-Cheng, Dong, Shuo-Han, Zhang, Qiu-Mei, Wu, Ting-Ting, Qiu, Li-Hong
• Format: Journal Article
• Language: English
• Published: England 01.09.2021
• Subjects: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Forests; Gammaproteobacteria - classification; Gammaproteobacteria - isolation & purification; Nucleic Acid Hybridization; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; China; phylogeny; telluris; Dyella; acidiphila
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004985

Cells of bacterial strains G9 and 7MK23 , isolated from forest soil samples collected from the Dinghushan Biosphere Reserve, Guangdong Province, PR China, were Gram-stain-negative, aerobic and rod-shaped. Strain G9 was motile with single polar flagellum and grew at 12-37 °C (optimum, 28 °C), pH 4.5-8.0 (optimum, pH 6.0-7.5) and in the presence of 0-3.5 % NaCl (optimum, 1.5%, w/v); while strain 7MK23 was non-motile and grew at 12-42 °C (optimum, 28-33 °C), pH 2.5-8.5 (optimum, pH 4.5-6.5) and NaCl levels of 0-1.0 % (optimum, 0-0.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates fell within the cluster of the genus . The closely related species (with a 16S rRNA gene sequence similarity >98.65%) of strain G9 were JS14-6 (99.0 %), . THG-B117 (98.8 %) and . DHC06 (98.7 %) while that of strain 7MK23 were . DHON07 (99.2 %) and . DHOC52 (99.1 %), but the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains G9 , 7MK23 and the closely related species listed above were in the ranges of 77.5-83.8 % and 22.0-27.0 %, much lower than the species demarcation lines of 95.5 and 70 %, respectively. Phylogenomic analyses using UBCG and Phylophlan also supported that these two strains represent two novel species of . The major fatty acids of strain G9 were iso-C , iso-C 9 and iso-C while that of strain 7MK23 were iso-C and anteiso-C . Ubiquinone-8 was the only respiratory quinone detected in both strains. The polar lipids of strain G9 consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and several unknown phospholipids, aminophospholipids, aminolipids and lipid while strain 7MK23 contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and several unknown phospholipids and aminophospholipids. The DNA G+C contents of strains G9 and 7MK23 were 64.7 and 63.4 mol%, respectively. On the basis of 16S rRNA gene sequence phylogenetic and phylogenomic analyses as well as phenotypic data obtained, we propose that strains G9 and 7MK23 represent two novel species of the genus , for which the names sp. nov. (type strain G9 =KACC 21725 =GDMCC 1.2132 ) and sp. nov. (type strain 7MK23 =KCTC 62739 =GDMCC 1.1446 ) are proposed.