Reclassification of Parvularcula flava as Aquisalinus luteolus nom. nov. and emended description of the genus Aquisalinus

• Published in: International journal of systematic and evolutionary microbiology Vol. 71; no. 10
• Main Authors: Ying, Jun-Jie, Wu, Zhi-Cheng, Fang, Yuan-Chun, Xu, Lin, Sun, Cong
• Format: Journal Article
• Language: English
• Published: England 01.10.2021
• Subjects: Alphaproteobacteria - classification; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Parvularcula flava; emended description; Aquisalinus flavus; Aquisalinus luteolus nom. nov; reclassification
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.005072

Parvularcula flava was proposed as a novel member of genus Parvularcula in 2016. Some time earlier, Aquisalinus flavus has been proposed as a novel species of a novel genus named Aquisalinus . When comparing the 16S rRNA gene sequences of type strains P. flava NH6-79 T and A. flavus D11M-2 T , they showed 97.9 % sequence identity, much higher than the sequence identities 92.7–94.3 % between P. flava NH6-79 T and type strains in the genus Parvularcula , indicating that the later proposed novel taxon Parvularcula flava need reclassification. The phylogenetic trees based on 16S rRNA gene sequences and genome sequences both showed that P. flava NH6-79 T and A. flavus D11M-2 T formed a separated branch away from strains in the genera Parvularcula , Marinicaulis and Amphiplicatus . The average amino acid identity and average nucleotide identity values of P. flava NH6-79 T and A. flavus D11M-2 T were 87.9 and 85.0 %, respectively, much higher than the values between P. flava NH6-79 T and other closely related type strains (54.3 %–58.1 % and 68.6–70.4 %, respectively). P. flava NH6-79 T and A. flavus D11M-2 T also contained summed feature 8 (C 18 : 1  ω 6 c and/or C 18 : 1  ω 7c) and C 16 : 0 as major fatty acids, distinguishing them from other closely related taxa. Based on the results of the phylogenetic, comparative genomic and phenotypic analyses, Parvularcula flava should be reclassified as Aquisalinus luteolus nom. nov. and the description of genus Aquisalinus is emended.