Effect of UV Light and Sodium Hypochlorite on Formation and Destruction of Pseudomonas fluorescens Biofilm In Vitro

Pseudomonas fluorescens is one of the first colonizers of bacterial biofilm in water systems and a member of opportunistic premise plumbing pathogens (OPPPs). The aim of this study was to examine the effect of UV light and sodium hypochlorite on the formation and destruction of mature P. fluorescens...

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Bibliographic Details
Published inProcesses Vol. 10; no. 10; p. 1901
Main Authors Zekanović, Melani Sigler, Begić, Gabrijela, Mežnarić, Silvestar, Jelovica Badovinac, Ivana, Krištof, Romana, Tomić Linšak, Dijana, Gobin, Ivana
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.10.2022
Subjects
Bacteria
Biofilms
By products
Chlorine
Destruction
Disinfection & disinfectants
Experiments
Exposure
Light
Mechanical cleaning
Microorganisms
Opportunist infection
Pathogens
Pseudomonas fluorescens
Sodium
Sodium hypochlorite
Swimming accidents & safety
Swimming pools
Ultraviolet radiation
Water supply
Italy
Switzerland
Japan
Germany
Croatia
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Summary:Pseudomonas fluorescens is one of the first colonizers of bacterial biofilm in water systems and a member of opportunistic premise plumbing pathogens (OPPPs). The aim of this study was to examine the effect of UV light and sodium hypochlorite on the formation and destruction of mature P. fluorescens biofilm on ceramic tiles. Planktonic bacteria or bacteria in mature biofilm were exposed to UV light (254 nm) for 5, 20 s. and to 0.4 mg/L sodium hypochlorite for 1 min. Mature biofilm was also exposed to increased concentration of sodium hypochlorite of 2 mg/L for 0.5, 1 and 2 h and combined with UV. Prolonged action of sodium hypochlorite and an increase in its concentration in combination with UV gave the best results in the inhibition of biofilm formation after the pre-treatment and destruction of mature biofilm. The effect of hyperchlorination in combination with UV radiation shows better results after a long exposure time, although even after 120 min there was no completely destroyed biofilm. Furthermore, the mechanism of the effect of combined methods should be explored as well as the importance of mechanical cleaning that is crucial in combating bacterial biofilm in swimming pools.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr10101901