Streptomyces salilacus sp. nov., an actinomycete isolated from a salt lake

• Published in: International journal of systematic and evolutionary microbiology Vol. 68; no. 5; pp. 1514 - 1518
• Main Authors: Luo, Xiao-Xia, Gao, Guang-Bin, Xia, Zhan-Feng, Chen, Zheng-Jun, Wan, Chuan-Xing, Zhang, Li-Li
• Format: Journal Article
• Language: English
• Published: England 01.05.2018
• Subjects: XinJiang; actinomycete; Streptomyces salilacus sp. nov
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.002703

The taxonomic position of a novel actinomycete, strain TRM 41337 , isolated from sediment of a salt lake, Xiaoerkule Lake, Xinjiang, China, was determined by a polyphasic approach. Strain TRM 41337 grew optimally at 28 °C and in the presence of 1 % (w/v) NaCl. It grew at up to pH 12. The whole-cell sugars of strain TRM 41337 were ribose and xylose. The diagnostic diamino acid contained ll-diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two other unidentified phospholipids. The predominant menaquinones were MK-9(H8), MK-9, MK-9(H4) and MK-9(H6). The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 1 H. Based on morphological and chemotaxonomic characteristics, the isolate was determined to belong to the genus Streptomyces. The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1498 nt) with representative strains showed that the strain consistently falls into a distinct phyletic lineage together with Streptomyces barkulensis DSM 42082 (97.48 % similarity) and a subclade consisting of Streptomyces fenghuangensis GIMN 4.003 (97.20 %), Streptomyces macrosporus NBRC 14748 (97.14 %) and Streptomyces radiopugnans R97 (97.01 %). On the basis of these data, strain TRM 41337 should be designated as a representative of a novel species of the genus Streptomyces, for which the name Streptomyces salilacus sp. nov. is proposed. The type strain is TRM 41337 (=CCTCC AA 2015030 =KCTC 39726 ).