Sediminibacterium roseum sp. nov., isolated from sewage sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 67; no. 11; pp. 4674 - 4679
• Main Authors: Song, Yali, Jia, Jianqiang, Liu, Dongmei, Choi, Lina, Wang, Gejiao, Li, Mingshun
• Format: Journal Article
• Language: English
• Published: England 01.11.2017
• Subjects: Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Phospholipids - chemistry; Phylogeny; Pigmentation; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sewage - microbiology; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Republic of Korea; Sediminibacterium; Chitionphagaceae; polyphasic taxonomy
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.002355

A novel bacterial strain, designated SYL130 , was isolated from the sewage sediment of a park in Busan, Korea. The strain was aerobic, producing orange colonies on R2A agar. Cells were single, Gram-stain-negative rods and were motile by gliding. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain SYL130 was most closely related to Sediminibacterium aquarii JCM 31013 (96.1 %). The major fatty acids (>5 % of the total) of strain SYL130 were iso-C15 : 0 (28.3 %), iso-C15 : 1 G (23.2 %), iso-C17 : 0 3-OH (9.6 %), anteiso-C15 : 0 (5.9 %) and iso-C16 : 0 3-OH (5.6 %). The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, two unidentified aminophospholipids and six unidentified polar lipids. The predominant respiratory quinone was MK-7. The DNA G+C content was 47.8 mol%. Strain SYL130 had clearly differential characteristics to related species including the temperature and pH ranges for growth, and being positive for l-arabinose and maltose, and negative for α-galactosidase activity. On the basis of phenotypic, chemotaxonomic and genotypic analyses, strain SYL130 represents a novel species of the genus Sediminibacterium, for which the name Sediminibacterium roseum sp. nov., is proposed. The type strain Sediminibacterium roseum is SYL130 (=KCTC 52860 =CCTCC AB 2017082 ).