Effect of reduced atmospheric pressures on the morphology and astaxanthin biosynthesis of microalga Haematococcus lacustris

• Published in: Biotechnology and bioprocess engineering Vol. 29; no. 6; pp. 1131 - 1140
• Main Authors: Kim, Sangui, Mahadi, Rendi, Narasimhan, Aditya Lakshmi, Christabel, Catherine, Yu, Hyoji, Kim, Eui-Jin, Oh, You-Kwan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.12.2024; 한국생물공학회
• Subjects: Algae; Aquatic microorganisms; Astaxanthin; Atmospheric pressure; Bioaccumulation; biochemical compounds; Biomolecules; Biosynthesis; canthaxanthin; Cell morphology; Cell size; cell structures; Cell viability; economic sustainability; Haematococcus; Haematococcus lacustris; Hydraulic pressure; Low pressure; Mars environment; Microalgae; Morphology; Pressure effects; Pressure reduction; Reactive oxygen species; 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-024-00145-y

The efficient production of the high-value antioxidant astaxanthin is crucial for economically viable biorefinement of the green microalga Haematococcus lacustris. In this study, we investigated the impact of reduced atmospheric pressure (0.8–0.2 bar) on cell morphology and astaxanthin biosynthesis in H. lacustris over 72 h. Astaxanthin content in microalgal cells subjected to depressurization at an optimal pressure of 0.6 bar for 48 h increased by 26.9 and 13.2% compared with that in initial cells and untreated controls, respectively. Furthermore, depressurization led to increased cell size and elevated levels of canthaxanthin (a major astaxanthin intermediate) and total fatty acids. Depressurized H. lacustris cells maintained high cell viability rates, ranging from 96.0 to 97.5% for 72 h. However, excessive or prolonged pressure reduction, particularly beyond 60 h or at 0.2–0.4 bar, hindered the ability of microalgae to synthesize astaxanthin. Astaxanthin accumulation under reduced pressure is likely associated with the formation of reactive oxygen species, which serve as signaling biomolecules triggering astaxanthin biosynthesis. These findings provide valuable insights into the biology and ketocarotenoid biosynthesis of microalgae under different hydraulic pressure conditions, as well as the responses of other biological components of life support systems required in low-pressure lunar and Martian environments.