Effects of acute stress on gene expression of splenic catecholamine biosynthetic enzymes in chronically stressed rats

The aim of this study was to examine how acute immobilization stress affects the concentrations of catecholamines in the plasma and the expression of the splenic catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-?-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNM...

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Bibliographic Details
Published inArchives of biological sciences Vol. 65; no. 1; pp. 183 - 189
Main Authors Gavrilovic, Ljubica, Stojiljkovic, Vesna, Kasapovic, Jelena, Pejic, Snezana, Todorovic, Ana, Pajovic, Snezana, Dronjak, Sladjana
Format Journal Article
LanguageEnglish
Published University of Belgrade, University of Novi Sad 2013
Subjects
acute immobilization
catecholamine
chronic social isolation
qRT-PCR
rats
spleen
Stress
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Summary:The aim of this study was to examine how acute immobilization stress affects the concentrations of catecholamines in the plasma and the expression of the splenic catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-?-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in chronically socially isolated rats. We found that acute immobilization increases the plasma catecholamine levels and splenic PNMT protein levels in chronically socially isolated rats. These results show that acute stress of chronically stressed animals activates the sympatho-adrenomedullary system and increases synthesis of splenic PNMT by 37%, both of which can modulate the immune function.
ISSN:0354-4664
1821-4339
DOI:10.2298/ABS1301183G