Bacterial toxicity of cyclodextrins: luminuous Escherichia coli as a model

The effect of various concentrations of natural and chemically modified cyclodextrins on the luminescence of an Escherichia coli suspension was investigated. All cyclodextrins were found to reduce, albeit to a varying degree, the luminescence level of the bacterial cells, thus suggesting a direct in...

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Bibliographic Details
Published inApplied microbiology and biotechnology Vol. 41; no. 5; p. 574
Main Authors Bar, R, Ulitzur, S
Format Journal Article
LanguageEnglish
Published Germany 01.07.1994
Subjects
Cyclodextrins - pharmacology
Dose-Response Relationship, Drug
Escherichia coli - drug effects
Luminescent Measurements
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Summary:The effect of various concentrations of natural and chemically modified cyclodextrins on the luminescence of an Escherichia coli suspension was investigated. All cyclodextrins were found to reduce, albeit to a varying degree, the luminescence level of the bacterial cells, thus suggesting a direct interaction between the cyclodextrins and cells. The inhibitory concentrations IC20 and IC50 of the various cyclodextrins were determined and taken to represent their toxicity effects upon the bacterial cells. Among the natural cyclodextrins, gamma- and alpha-CD interfered minimally with the bacterial luminescence and consequently were essentially non-toxic. The following descending order of toxicity was observed: beta-CD >> alpha-CD > gamma-CD. Among the chemically modified cyclodextrins, Dimeb was clearly toxic while Trimeb and the hydroxylated derivatives (hydroxypropyl-alpha-CD, HPACD; -beta-CD, HPBCD; -gamma-CD, HPGCD) were essentially non-toxic. The following descending order of toxicity was observed: Dimeb >> HPBCD > Trimeb > HPACD > HPGCD.
ISSN:0175-7598
1432-0614
DOI:10.1007/bf00178492