Erythrocytes of the common carp are immune sentinels that sense pathogen molecular patterns, engulf particles and secrete pro-inflammatory cytokines against bacterial infection

• Published in: Frontiers in immunology Vol. 15; p. 1407237
• Main Authors: Majstorović, Jovana, Kyslík, Jiří, Klak, Katarzyna, Maciuszek, Magdalena, Chan, Justin T H, Korytář, Tomáš, Holzer, Astrid S
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 14.06.2024
• Subjects: Aeromonas hydrophila (A. hydrophila); Aeromonas hydrophila - immunology; Animals; bacteria; Carps - immunology; Carps - microbiology; Cyprinus carpio; cytokines; Cytokines - immunology; Cytokines - metabolism; Erythrocytes - immunology; Erythrocytes - metabolism; Fish Diseases - immunology; Fish Diseases - microbiology; Gram-Negative Bacterial Infections - immunology; Immunity, Innate; Pathogen-Associated Molecular Pattern Molecules - immunology; Phagocytosis - immunology; red blood cell (RBC); teleost fish; engulfment; bacteria; inflammation; Cyprinus carpio; cytokines; teleost fish; Aeromonas hydrophila (A. hydrophila); red blood cell (RBC)
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2024.1407237

Red blood cells (RBCs), also known as erythrocytes, are underestimated in their role in the immune system. In mammals, erythrocytes undergo maturation that involves the loss of nuclei, resulting in limited transcription and protein synthesis capabilities. However, the nucleated nature of non-mammalian RBCs is challenging this conventional understanding of RBCs. Notably, in bony fishes, research indicates that RBCs are not only susceptible to pathogen attacks but express immune receptors and effector molecules. However, given the abundance of RBCs and their interaction with every physiological system, we postulate that they act in surveillance as sentinels, rapid responders, and messengers. We performed a series of experiments with RBCs exposed to , as well as laboratory infections using different concentrations of bacteria. qPCR revealed that RBCs express genes of several inflammatory cytokines. Using cyprinid-specific antibodies, we confirmed that RBCs secreted tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ). In contrast to these indirect immune mechanisms, we observed that RBCs produce reactive oxygen species and, through transmission electron and confocal microscopy, that RBCs can engulf particles. Finally, RBCs expressed and upregulated several putative toll-like receptors, including and , in response to infection . Overall, the RBC repertoire of pattern recognition receptors, their secretion of effector molecules, and their swift response make them immune sentinels capable of rapidly detecting and signaling the presence of foreign pathogens. By studying the interaction between a bacterium and erythrocytes, we provide novel insights into how the latter may contribute to overall innate and adaptive immune responses of teleost fishes.