Development of Mouse Monoclonal Antibodies Against Human Amyloid Fibril Proteins for Diagnostic and Research Purposes

Commercial antibodies against varying proteins are often not optimal for identification of proteins in their amyloid fibril forms. Reasons can be the different conformation but also a variety of modifications like N- or C-terminal truncation. Therefore, development of own monoclonal antibodies again...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1779; p. 401
Main Authors Westermark, Gunilla T, Ihse, Elisabet, Westermark, Per
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Amyloidosis - diagnosis
Amyloidosis - genetics
Amyloidosis - metabolism
Animals
Antibodies, Monoclonal, Murine-Derived - metabolism
Epitopes - metabolism
Humans
Hybridomas - cytology
Hybridomas - metabolism
Mice
Prealbumin - chemistry
Prealbumin - genetics
Prealbumin - immunology
Rabbits
Immunohistochemistry
Western blot
Degraded amyloid
Transthyretin
Hybridoma
Amyloid
Fibril
Online AccessGet more information

Cover

More Information
Summary:Commercial antibodies against varying proteins are often not optimal for identification of proteins in their amyloid fibril forms. Reasons can be the different conformation but also a variety of modifications like N- or C-terminal truncation. Therefore, development of own monoclonal antibodies against amyloid fibril proteins may be advantageous. This chapter gives suggestions of how to be successful in such approaches.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7816-8_24