Unveiling the Potential of CuO and Cu2O Nanoparticles against Novel Copper-Resistant Pseudomonas Strains: An In-Depth Comparison

• Published in: Nanomaterials (Basel, Switzerland) Vol. 14; no. 20; p. 1644
• Main Authors: Havryliuk, Olesia, Rathee, Garima, Blair, Jeniffer, Hovorukha, Vira, Tashyrev, Oleksandr, Morató, Jordi, Pérez, Leonardo M., Tzanov, Tzanko
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 13.10.2024; MDPI
• Subjects: Antibiotics; Antimicrobial agents; Bacteria; Biofilms; Cell morphology; Copper; copper oxide nanoparticles; Copper oxides; Fourier transforms; Glycerol; Heavy metals; High resolution electron microscopy; Infrared spectroscopy; Membranes; metal-resistant bacteria; Microorganisms; Morphology; Nanomaterials; Nanoparticles; Nanotechnology; Oxidative stress; Pathogens; Photoelectron spectroscopy; Photoelectrons; Pseudomonas; Pseudomonas sp; sonochemical synthesis; Spectrum analysis; Strains (organisms); Toxicity; Transmission electron microscopy; wild-type bacteria control; X ray photoelectron spectroscopy; United States > US; Spain
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano14201644

Four novel Pseudomonas strains with record resistance to copper (Cu2+) previously isolated from ecologically diverse samples (P. lactis UKR1, P. panacis UKR2, P. veronii UKR3, and P. veronii UKR4) were tested against sonochemically synthesised copper-oxide (I) (Cu2O) and copper-oxide (II) (CuO) nanoparticles (NPs). Nanomaterials characterisation by X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and High-Resolution Transmission Electron Microscopy (HRTEM) confirmed the synthesis of CuO and Cu2O NPs. CuO NPs exhibited better performance in inhibiting bacterial growth due to their heightened capacity to induce oxidative stress. The greater stability and geometrical shape of CuO NPs were disclosed as important features associated with bacterial cell toxicity. SEM and TEM images confirmed that both NPs caused membrane disruption, altered cell morphology, and pronounced membrane vesiculation, a distinctive feature of bacteria dealing with stressor factors. Finally, Cu2O and CuO NPs effectively decreased the biofilm-forming ability of the Cu2+-resistant UKR strains as well as degraded pre-established biofilm, matching NPs’ antimicrobial performance. Despite the similarities in the mechanisms of action revealed by both NPs, distinctive behaviours were also detected for the different species of wild-type Pseudomonas analysed. In summary, these findings underscore the efficacy of nanotechnology-driven strategies for combating metal tolerance in bacteria.