Specific cytosol-binding protein for 1,25-dihydroxyvitamin D3 in rat intestine

Cytosol prepared from intestinal homogenates of vitamin D3-deficient rats demonstrate a 3.2 S protein having high affinity and low capacity for 1,25-dihydroxyvitamin D3. Appearance of the protein is dependent upon the presence of 0.3 M potassium chloride and dithiothreitol in the homogenization buff...

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Published inThe Journal of biological chemistry Vol. 252; no. 13; pp. 4501 - 4505
Main Authors Kream, B E, Yamada, S, Schnoes, H K, DeLuca, H F
Format Journal Article
LanguageEnglish
Published United States American Society for Biochemistry and Molecular Biology 10.07.1977
Subjects
Animals
Binding, Competitive
Blood Proteins - metabolism
Carrier Proteins - blood
Carrier Proteins - metabolism
Centrifugation, Density Gradient
Cytosol - metabolism
Dihydroxycholecalciferols - metabolism
Hydroxycholecalciferols - metabolism
In Vitro Techniques
Intestinal Mucosa - metabolism
Intestinal Mucosa - ultrastructure
Male
Rats
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Summary:Cytosol prepared from intestinal homogenates of vitamin D3-deficient rats demonstrate a 3.2 S protein having high affinity and low capacity for 1,25-dihydroxyvitamin D3. Appearance of the protein is dependent upon the presence of 0.3 M potassium chloride and dithiothreitol in the homogenization buffer. A 6 S protein having greater affinity for 25-hydroxyvitamin D3 than 1,25-dihydroxyvitamin D3 is observed using all conditions and is increased by the addition of rat serum to cytosol. The 3.2 S protein is neither a serum contaminant nor a component of the 6 S protein and is probably not a consituent of tissues which which are not responsive to vitamin D3.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)40189-X