Microbial aromatic amino acid metabolism is modifiable in fermented food matrices to promote bioactivity

• Published in: Food chemistry Vol. 454; p. 139798
• Main Authors: Kasperek, Mikaela C., Velasquez Galeas, Adriana, Caetano-Silva, Maria Elisa, Xie, Zifan, Ulanov, Alexander, La Frano, Michael, Devkota, Suzanne, Miller, Michael J., Allen, Jacob M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2024
• Subjects: AhR; Amino Acids, Aromatic - metabolism; Bioactivity; Fermentation; Fermented food; Fermented Foods - analysis; Fermented Foods - microbiology; Humans; Lactates - metabolism; Lactobacillales - metabolism; Metabolites; Microbiota; Receptors, Aryl Hydrocarbon - metabolism; Microbiota; AhR; Bioactivity; Metabolites; Fermented food
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2024.139798

Ingestion of fermented foods impacts human immune function, yet the bioactive food components underlying these effects are not understood. Here, we interrogated whether fermented food bioactivity relates to microbial metabolites derived from aromatic amino acids, termed aryl-lactates. Using targeted metabolomics, we established the presence of aryl-lactates in commercially available fermented foods. After pinpointing fermented food-associated lactic acid bacteria that produce high levels of aryl-lactates, we identified fermentation conditions to increase aryl-lactate production in food matrices up to 5 × 103 fold vs. standard fermentation conditions. Using ex vivo reporter assays, we found that food matrix conditions optimized for aryl-lactate production exhibited enhanced agonist activity for the human aryl-hydrocarbon receptor (AhR) as compared to standard fermentation conditions and commercial products. Reduced microbial-induced AhR activity has emerged as a hallmark of many chronic inflammatory diseases, thus we envision strategies to enhance AhR bioactivity of fermented foods to be leveraged to improve human health. •Fermented foods contain bioactive microbe-derived aromatic amino acid metabolites.•Aromatic amino acid metabolism can be manipulated to increase aryl-lactates.•Optimized food ferments increase human aryl-hydrocarbon receptor activity.