Effects of SOCS 1/3 gene silencing on the expression of C/EBPα and PPARγ during differentiation and maturation of rat preadipocytes

• Published in: Pediatric research Vol. 73; no. 3; pp. 263 - 267
• Main Authors: Zheng, Rui-Dan, Liao, Li-Hong, Ye, Juan, Wang, Cheng-Bin, Gao, Jin-Zhi, Ying, Yan-Qin, Ning, Qin, Luo, Xiao-Ping
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2013
• Subjects: 631/136/142; 631/208/176/2016; 631/208/199; 692/699/2743/1530; Adipocytes - physiology; Animals; basic-science-investigation; Blotting, Western; CCAAT-Enhancer-Binding Proteins - metabolism; Cell Differentiation - physiology; Cell Growth Processes - physiology; DNA Primers - genetics; Gene Silencing; Insulin Resistance - physiology; Medicine; Medicine & Public Health; Pediatric Surgery; Pediatrics; PPAR gamma - metabolism; Rats; Real-Time Polymerase Chain Reaction; Suppressor of Cytokine Signaling 1 Protein; Suppressor of Cytokine Signaling 3 Protein; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism
• ISSN: 0031-3998; 1530-0447; 1530-0447
• DOI: 10.1038/pr.2012.190

Background: Suppressor of cytokine signaling-1 and -3 (SOCS-1 and SOCS-3) are two important negative regulators in the insulin-signaling pathway, and their overexpression may aggravate insulin resistance. Subjects with insulin resistance are often obese and have increased expressions of SOCS-1 and SOCS-3. We speculated that SOCS-1 and SOCS-3 may be involved in abnormal deposition of adipose tissues during insulin resistance. Methods: A catch-up growth intrauterine growth retardation (CG-IUGR) rat model with insulin resistance was established; mRNA and protein expression of SOCS-1, SOCS-3, the CCAAT/enhancer binding protein (C/EBPα), and peroxisome proliferator-activated receptor (PPARγ) in adipose tissue were measured by real-time PCR and western blot; plasmids carrying small hairpin RNAs (shRNAs) targeting the SOCS-1 and SOCS-3 genes were constructed and transfected into preadipocytes, which were then induced to mature. At 72 h after differentiation was induced, the expressions of C/EBPα and PPARγ, two important molecules promoting the differentiation of preadipocytes, were detected. Results: Expressions of SOCS-1, SOCS-3, C/EBPα, and PPARγ were markedly increased in adipose tissues of CG-IUGR rats, whereas the expressions of C/EBPα and PPARγ were significantly reduced after gene silencing of SOCS-1 or SOCS-3 in adipocytes. Conclusion: Overexpression of SOCS-1 and SOCS-3 may enhance the expression of C/EBPα and PPARγ, resulting in abnormal deposition of adipose tissues during insulin resistance.