β-Hydroxy-β-methylbutyrate modulates lipid metabolism in adipose tissues of growing pigs

• Published in: Food & function Vol. 9; no. 9; pp. 4836 - 4846
• Main Authors: Duan, Yehui, Zhang, Lingyu, Li, Fengna, Guo, Qiuping, Long, Cimin, Yin, Yulong, Kong, Xiangfeng, Peng, Mijun, Wang, Wence
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 19.09.2018
• Subjects: Adiponectin; Adiponectin - blood; Adipose tissue; Adipose Tissue, White - growth & development; Adipose Tissue, White - immunology; Adipose Tissue, White - metabolism; Adiposity; Amino Acids, Branched-Chain - blood; AMP-Activated Protein Kinases - metabolism; Animals; Biomarkers - blood; Body fat; China; Crosses, Genetic; Diet; Diet - veterinary; Dietary supplements; Fatty acids; Gene expression; Gene Expression Regulation, Developmental; Interleukin 15; Keto Acids - administration & dosage; Leucine; Lipid Metabolism; Lipids; Lipolysis; Metabolism; Metabolites; Mitochondria; mRNA; Muscles; Oxidation; Phosphorylation; Plasma; Protein Processing, Post-Translational; Protein Subunits - metabolism; Random Allocation; Sus scrofa; Swine; TOR protein; TOR Serine-Threonine Kinases - metabolism; Valerates - administration & dosage; Weight Gain; Weight reduction; China
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/c8fo00898a

Background : The effects and roles of the leucine (Leu) metabolite β-hydroxy-β-methylbutyrate (HMB) in lipid metabolism in adipose tissues of pigs are still unknown. Objectives : This study was conducted to investigate the effects of excess Leu versus HMB on growth, carcass traits, and lipid metabolism in adipose tissues of growing pigs. Methods and results : Compared to control, the Leu/HMB group significantly increased/reduced weight of total fat mass, respectively, with a concurrent increase of serum adiponectin concentration ( P < 0.05). Moreover, dietary HMB supplementation regulated the expression of genes involved in adipose tissue function, accompanied by increases/decreases in the phosphorylation of AMPKα/mTOR in perirenal adipose tissue, respectively ( P < 0.05). Serum IL-15 concentration and the mRNA abundance of IL-15, PGC-1α, and NRF-1 were also increased in the HMB group ( P < 0.05). Conclusions : HMB supplementation can regulate adipose tissue function including fatty acid oxidation, lipolysis, and adipokine secretion. These effects may be partly mediated by AMPKα-mTOR pathway and associated with mitochondrial biogenesis, the AMPK-PGC-1α axis, and myokines secreted by muscle tissues. Background : The effects and roles of the leucine (Leu) metabolite β-hydroxy-β-methylbutyrate (HMB) in lipid metabolism in adipose tissues of pigs are still unknown.