Aqueous Humor Penetration and Biological Activity of Moxifloxacin 0.5% Ophthalmic Solution Alone or with Dexamethasone 0.1

• Published in: Journal of ocular pharmacology and therapeutics Vol. 33; no. 2; p. 98
• Main Authors: Gomes, Rachel L R, Viana, Rodrigo Galvão, Melo, Jr, Luiz Alberto S, Cruz, Alessandro Carvalho, Suenaga, Eunice Mayumi, Kenyon, Kenneth R, Campos, Mauro
• Format: Journal Article
• Language: English
• Published: United States 01.03.2017
• Subjects: Administration, Topical; Aged; Aqueous Humor - drug effects; Aqueous Humor - microbiology; Chromatography, High Pressure Liquid; Dexamethasone - administration & dosage; Dexamethasone - analysis; Dexamethasone - pharmacology; Enterococcus - drug effects; Female; Fluoroquinolones - administration & dosage; Fluoroquinolones - analysis; Fluoroquinolones - pharmacology; Humans; Male; Microbial Sensitivity Tests; Ophthalmic Solutions - administration & dosage; Ophthalmic Solutions - analysis; Ophthalmic Solutions - pharmacology; Prospective Studies; Staphylococcus - drug effects; Tandem Mass Spectrometry; steroids; corneal permeability; aqueous humor; fluoroquinolone ophthalmic solutions; antibiotics
• ISSN: 1557-7732
• DOI: 10.1089/jop.2016.0126

To compare aqueous humor concentrations of topically applied moxifloxacin 0.5% ophthalmic solution alone or in combination with dexamethasone 0.1% and to correlate these concentrations with the minimum inhibitory concentrations (MICs) for common endophthalmitis-causing organisms. Sixty-eight patients undergoing routine phacoemulsification with intraocular lens implantation received either moxifloxacin 0.5% alone or moxifloxacin 0.5% combined with dexamethasone. For both groups, 1 drop of the test solution was instilled 4 times daily 1 day preoperatively and 1 drop 1 h preoperatively. An aqueous humor sample obtained immediately before paracentesis was submitted to high-performance liquid chromatography-tandem mass spectrometry to determine the moxifloxacin concentration. The mean concentrations of moxifloxacin were 986.6 ng/mL in the moxifloxacin with dexamethasone group and 741.3 ng/mL in the moxifloxacin group (P = 0.13). Moxifloxacin concentrations of all samples exceeded the MICs for Staphylococcus epidermidis, S. aureus, and Streptococcus pneumoniae. All samples in the moxifloxacin with dexamethasone group and 94% in the moxifloxacin group achieved the MIC for Enterococcus species. For quinolone-resistant S. aureus, the MIC was achieved in 29% in the moxifloxacin with dexamethasone group and 9% in the moxifloxacin group (P = 0.06). Aqueous humor moxifloxacin concentrations were higher when topically administrated in combination with dexamethasone compared to the moxifloxacin alone. However, this difference was not statistically significant. Nevertheless, the MICs of the most common pathogens associated with endophthalmitis were exceeded in both study groups.