Molecular typing of mcr-1 Escherichia coli isolates from pigs and farm environment based on fumC and fimH alleles

• Published in: Future microbiology Vol. 18; no. 8; pp. 481 - 488
• Main Authors: Oliveira, Gabriela S, Lentz, Silvia Am, Wink, Priscila L, Martins, Andreza F
• Format: Journal Article
• Language: English
• Published: England 01.05.2023
• Subjects: Adhesins, Escherichia coli - genetics; Alleles; Animals; Anti-Bacterial Agents - pharmacology; Colistin - pharmacology; Escherichia coli; Escherichia coli Infections - genetics; Escherichia coli Infections - veterinary; Escherichia coli Proteins - genetics; Farms; Fimbriae Proteins - genetics; Microbial Sensitivity Tests; Multilocus Sequence Typing; Phylogeny; Swine; clonal expansion; horizontal gene transfer; pig farming; CH typing; Escherichia coli; mcr-1
• ISSN: 1746-0913; 1746-0921
• DOI: 10.2217/fmb-2022-0173

The dissemination of polymyxin resistance represents a significant threat to public health. Sequence-based typing was performed by 53 isolates using /  (CH) genes to characterize clones spreading from pig farming. Furthermore, 12 isolates had their whole genome sequenced for phylogenetic study. The isolates were classified into 22 distinct CH types, and two novel CH types (CH41-1578 and CH4-1579) and one sequence type (ST12652) was also described. According to phylogenetic study, both multilocus sequence typing and CH methods grouped the isolates similarly. Our findings suggest that the dissemination of the gene in pig farming has occurred mainly by horizontal gene transfer, and CH typing proved to be a good tool to characterize clones.