Annealing temperature influences the cytocompatibility, bactericidal and bioactive properties of green synthesised TiO2 nanocomposites

• Published in: Chemical papers Vol. 76; no. 9; pp. 5369 - 5388
• Main Authors: Govindasamy, G. Ambarasan, Mydin, Rabiatul Basria S. M. N., Harun, Nor Hazliana, Effendy, Wan Nuramiera Faznie Wan Eddis, Sreekantan, Srimala
• Format: Journal Article
• Language: English
• Published: Warsaw Versita 01.09.2022; Springer Nature B.V
• Subjects: Anatase; Annealing; Biochemistry; Biocompatibility; Biological activity; Biotechnology; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chlorine; Crystallites; E coli; In vivo methods and tests; Industrial Chemistry/Chemical Engineering; Magnesium; Materials Science; Medicinal Chemistry; Nanocomposites; Original Paper; Pseudomonas aeruginosa; Titanium dioxide; Wound healing; Annealing temperature; Cytocompatibility; Titanium dioxide nanoparticles; Green synthesised nanoparticles; Bactericidal agent
• ISSN: 0366-6352; 1336-9075; 2585-7290
• DOI: 10.1007/s11696-022-02230-z

Annealing is a crucial functional parameter relevant to the green synthesis and bactericidal properties of TiO 2 nanocomposites (TiO 2 -NPs). In this work, the effect of the annealing temperature on the physicochemical, bactericidal and cytocompatibility properties of TiO 2 -NPs obtained from Calotropis gigantea was comprehensively studied. Results indicated that amorphous-phase TiO 2 -NPs were transformed into the anatase phase at 500 °C with a crystallite size of 40.9 nm and MIC of 100 mg/mL towards Staphylococcus aureus . Whereas TiO 2 -NPs annealed at 400 °C demonstrated no bacterial reduction, TiO 2 -NPs annealed at 500 °C showed a moderate zone of inhibition towards Escherichia coli and Pseudomonas aeruginosa . Findings from this study found that TiO 2 -500C nanocomposites concentration at 100 mg/mL does not inhibit fibroblast cells proliferation activity after 24 h treatment. The plant-mediated nano-sized cubic and spherical anatase TiO 2 -NPs encapsulated bioactive green elements, such as carbon, sodium, magnesium, chlorine, potassium, calcium and sulphur, from the C . gigantea extract, ultimately leading to versatile and eco-friendly bactericidal agents with wound-healing properties. Further studies involving in vivo are needed to support this work.