Purification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic precursors: preliminary results

CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling....

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Bibliographic Details
Published inJournal of hematotherapy Vol. 2; no. 2; p. 259
Main Authors Feron, C, Gothot, A, Grosdent, J C, van Cauwenberge, J R, Schaaps, J P, Beguin, Y, Paulus, J M
Format Journal Article
LanguageEnglish
Published United States 1993
Subjects
Avidin
Biotin
Cell Differentiation
Cell Division - drug effects
Cell Separation - instrumentation
Culture Media, Serum-Free
Cytokines - pharmacology
Fetal Blood - cytology
Fluorescent Dyes
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Humans
Immunosorbent Techniques
Infant, Newborn
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Summary:CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling. The results indicated that the avidin column produced the highest purity of CD34-positive cells, and that immature blast cells could be expanded in serum-free culture. Preliminary results suggested that the four fluorochrome labeling technique may provide useful information on the lineage commitment of cord blood precursor and blast cells.
ISSN:1061-6128
2168-6556
DOI:10.1089/scd.1.1993.2.259