Effect of Different Extractant Concentrations on the Content of Biologically Active Substances in Motherwort Tincture

• Published in: Razrabotka i registraciâ lekarstvennyh sredstv (Online) Vol. 9; no. 4; pp. 72 - 78
• Main Authors: Rogozhnikova, E. P., Mizina, P. G., Mardanly, S. G.
• Format: Journal Article
• Language: English; Russian
• Published: LLC Center of Pharmaceutical Analytics (LLC «CPHA») 26.11.2020
• Subjects: extractant; flavonoids; fractional maceration; iridoids; maceration; modified fractional maceration; motherwort tincture; percolation
• ISSN: 2305-2066; 2658-5049
• DOI: 10.33380/2305-2066-2020-9-4-72-78

Introduction . This article considers the ways to optimize the motherwort tincture formulation while reducing the content of ethyl alcohol in the finished product. Qualitative and quantitative tincture test items depending on extraction method used and extractant concentration have been studied. Aim . To study the possibility of reducing the content of ethyl alcohol in motherwort tincture. Materials and methods . Comparison of quantitative and qualitative test items of tincture samples prepared by percolation, maceration, fractional maceration, modified fractional maceration is carried out. Quantitative comparative test items: dry residue, ethyl alcohol content, microbiological purity, quantitation, qualitative reactions – the presence of phenolic compounds, flavonoids in the samples (qualitative reactions, HPLC), iridoids (TLC) was used. Results and discussion . The developed method of modified fractional maceration consists in extracting herb materials with a more concentrated extractant (in the study, 80 %, 60 %, 40 % ethyl alcohol), then the subsequent extraction of herb materials with purified water. Comparison of the presence of different classes of biologically active substances in tinctures obtained by different technological methods and in infusions is carried out. Conclusion . It has been established that the most rational method is to obtain a tincture by modified fractional maceration method with ethyl alcohol content NLT 35 % in the finished product. The obtained results can be used in pharmaceutical industry for drug production by extractive methods, as well as for studies on technology optimization for obtaining extractive drugs from medicinal herb materials.