Medium- and long-chain 3-hydroxymonocarboxylic acids: analysis by gas chromatography combined with mass spectrometry

Medium- and long-chain 3-hydroxymonocarboxylic acids represent intermediates in the beta-oxidation of fatty acids: they accumulate in the plasma of patients with an inherited deficiency of long-chain 3-hydroxyacylcoenzyme A dehydrogenase. 3-Hydroxy acids with chain lengths varying from 6 to 16 were...

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Bibliographic Details
Published inBiomedical chromatography Vol. 5; no. 4; p. 161
Main Authors Dorland, L, Ketting, D, Bruinvis, L, Duran, M
Format Journal Article
LanguageEnglish
Published England 01.07.1991
Subjects
Carboxylic Acids - analysis
Carboxylic Acids - metabolism
Fatty Acids - metabolism
Gas Chromatography-Mass Spectrometry - instrumentation
Gas Chromatography-Mass Spectrometry - methods
Humans
Plasma - chemistry
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Summary:Medium- and long-chain 3-hydroxymonocarboxylic acids represent intermediates in the beta-oxidation of fatty acids: they accumulate in the plasma of patients with an inherited deficiency of long-chain 3-hydroxyacylcoenzyme A dehydrogenase. 3-Hydroxy acids with chain lengths varying from 6 to 16 were synthesized by a Reformatzky reaction. Capillary gas chromatography of the pertrimethylsilyl derivatives was performed on a CP-Sil 19 CB column, coupled to a quadrupole mass spectrometer in the electron impact mode. Calculation of the retention indices showed that the separation of the 3-hydroxy acids from the homologous straight-chain fatty acids may be troublesome, stressing the need for mass spectrometric identification.
ISSN:0269-3879
DOI:10.1002/bmc.1130050405