Polynucleotide helix geometry and stability. Spectroscopic, antigenic and interferon-inducing properties of deoxyribose-, ribose-, or 2'-deoxy-2'-fluororibose-containing duplexes of poly(inosinic acid) . poly(cytidylic acid)

• Published in: The Journal of biological chemistry Vol. 257; no. 4; pp. 1924 - 1928
• Main Authors: Kakiuchi, N, Marck, C, Rousseau, N, Leng, M, De Clerq, E, Guschlbauer, W
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 25.02.1982
• Subjects: Animals; Cells, Cultured; Circular Dichroism; Deoxyribose - analogs & derivatives; Epitopes - analysis; Immunodiffusion; Interferons - biosynthesis; Kidney - drug effects; Nucleic Acid Conformation; Nucleic Acid Denaturation; Poly I - immunology; Poly I - pharmacology; Poly I-C - immunology; Poly I-C - pharmacology; Rabbits; Radioimmunoassay; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Temperature
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(19)68127-5

Circular dichroism, absorbance temperature profiles, antigenic properties, and interferon-inducing capacity of the nine double-stranded complexes between poly(inosinic acid) and poly(cytidylic acid) [(rI)n . (rC)n] and their deoxy- and 2'-deoxy-2'-fluoro-analogues were studied. The complexes containing only ribose or 2'-fluororibose chains showed similar CD spectra and thermal stabilities. Anti-(rI)n . (rC)n antibodies were well recognized by the duplexes containing 2'-fluororibose in either one strand. These two duplexes were also efficient interferon inducers. Presence of fluororibose in both strands decreased the affinity of anti-(rI)n . (rC)n antibodies slightly and abolished interferon-inducing activity. All polydeoxyriboside-containing complexes showed CD spectra significantly different from the previous group; they showed also 40- to 100-times lower affinity for the anti-(rI)n . (rC)n antibodies, and did not induce interferon. It is concluded that the structure of (rI)n . (rC)n, an A-type helix, is little perturbed by substitution of one or both strands by 2'-fluororibose. Substitution by deoxyribose in either strand considerably changes the structure of the helices. The hybrids containing poly(deoxycytidylic acid) retain at least some of the structural features of the B-type helix poly(deoxyinosinic acid) . poly(deoxycytidylic acid).