Polynucleotide helix geometry and stability. Spectroscopic, antigenic and interferon-inducing properties of deoxyribose-, ribose-, or 2'-deoxy-2'-fluororibose-containing duplexes of poly(inosinic acid) . poly(cytidylic acid)
Circular dichroism, absorbance temperature profiles, antigenic properties, and interferon-inducing capacity of the nine double-stranded complexes between poly(inosinic acid) and poly(cytidylic acid) [(rI)n . (rC)n] and their deoxy- and 2'-deoxy-2'-fluoro-analogues were studied. The complex...
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Published in | The Journal of biological chemistry Vol. 257; no. 4; pp. 1924 - 1928 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
25.02.1982
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Subjects | |
Online Access | Get full text |
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Summary: | Circular dichroism, absorbance temperature profiles, antigenic properties, and interferon-inducing capacity of the nine double-stranded
complexes between poly(inosinic acid) and poly(cytidylic acid) [(rI)n . (rC)n] and their deoxy- and 2'-deoxy-2'-fluoro-analogues
were studied. The complexes containing only ribose or 2'-fluororibose chains showed similar CD spectra and thermal stabilities.
Anti-(rI)n . (rC)n antibodies were well recognized by the duplexes containing 2'-fluororibose in either one strand. These
two duplexes were also efficient interferon inducers. Presence of fluororibose in both strands decreased the affinity of anti-(rI)n
. (rC)n antibodies slightly and abolished interferon-inducing activity. All polydeoxyriboside-containing complexes showed
CD spectra significantly different from the previous group; they showed also 40- to 100-times lower affinity for the anti-(rI)n
. (rC)n antibodies, and did not induce interferon. It is concluded that the structure of (rI)n . (rC)n, an A-type helix, is
little perturbed by substitution of one or both strands by 2'-fluororibose. Substitution by deoxyribose in either strand considerably
changes the structure of the helices. The hybrids containing poly(deoxycytidylic acid) retain at least some of the structural
features of the B-type helix poly(deoxyinosinic acid) . poly(deoxycytidylic acid). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(19)68127-5 |