Improved Mitochondrial and Methylglyoxal-Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells

• Published in: Journal of cellular physiology Vol. 231; no. 9; pp. 2014 - 2025
• Main Authors: Falone, Stefano, Santini Jr, Silvano, di Loreto, Silvia, Cordone, Valeria, Grannonico, Marta, Cesare, Patrizia, Cacchio, Marisa, Amicarelli, Fernanda
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.09.2016
• Subjects: Cell Line, Tumor; Cell Proliferation - drug effects; Energy Metabolism - drug effects; Homeostasis - drug effects; Homeostasis - physiology; Humans; Magnetic Fields; Mitochondria - drug effects; Mitochondria - pathology; Neuroblastoma - drug therapy; Neuroblastoma - pathology; Neurons - drug effects; Neurons - pathology; Pyruvaldehyde - pharmacology
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.25310

Extremely low frequency magnetic fields (ELF‐MF) are common environmental agents that are suspected to promote later stages of tumorigenesis, especially in brain‐derived malignancies. Even though ELF magnetic fields have been previously linked to increased proliferation in neuroblastoma cells, no previous work has studied whether ELF‐MF exposure may change key biomolecular features, such as anti‐glycative defence and energy re‐programming, both of which are currently considered as crucial factors involved in the phenotype and progression of many malignancies. Our study investigated whether the hyperproliferation that is induced in SH‐SY5Y human neuroblastoma cells by a 50 Hz, 1 mT ELF magnetic field is supported by an improved defense towards methylglyoxal (MG), which is an endogenous cancer‐static and glycating α‐oxoaldehyde, and by rewiring of energy metabolism. Our findings show that not only the ELF magnetic field interfered with the biology of neuron‐derived malignant cells, by de‐differentiating further the cellular phenotype and by increasing the proliferative activity, but also triggered cytoprotective mechanisms through the enhancement of the defense against MG, along with a more efficient management of metabolic energy, presumably to support the rapid cell outgrowth. Intriguingly, we also revealed that the MF‐induced bioeffects took place after an initial imbalance of the cellular homeostasis, which most likely created a transient unstable milieu. The biochemical pathways and molecular targets revealed in this research could be exploited for future approaches aimed at limiting or suppressing the deleterious effects of ELF magnetic fields. J. Cell. Physiol. 231: 2014–2025, 2016. © 2016 Wiley Periodicals, Inc.