Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement: Part I. Optimization of derivated histamine detection with coated-plates using optimal design

Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (< 300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. T...

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Published inChemometrics and intelligent laboratory systems Vol. 80; no. 2; pp. 176 - 185
Main Authors Claeys-Bruno, M., Vandenabeele-Trambouze, O., Sergent, M., Geffard, M., Bodet, D., Dobrijevic, M., Commeyras, A., Phan Tan Luu, R.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.02.2006
Elsevier
Subjects
Antibodies
Astrophysics
Cosmology and Extra-Galactic Astrophysics
Derivatization
Experimental design
Histamine analysis
Hybrid design
Physics
Sciences of the Universe
Hybrid design
Antibodies
Histamine analysis
Derivatization
Experimental design
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Summary:Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (< 300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. The development of a quantitative method needs an optimization of both detection and derivatization step. Experimental design methodology has been applied to optimize the conditions of the detection of the derivatized histamine. Methods and results were closely related.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0169-7439
1873-3239
DOI:10.1016/j.chemolab.2005.06.006