Architecture mechanics mediated osteogenic progression in bone regeneration of artificial scaffolds

Scaffold architecture exerts a considerable influence on the osteogenic effect through stress transmission, as the deformation of scaffolds alters the mechanical microenvironment of cells adhering to scaffold surface. Despite extensive research on bone regeneration influenced by scaffold architectur...

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Published inScience advances Vol. 11; no. 29; p. eadv8804
Main Authors Long, Si-Yu, Fu, Ya-Jun, Zhang, Zheng-Min, Tang, Rui, Yu, Peng, Yang, Wei
Format Journal Article
LanguageEnglish
Published United States 18.07.2025
Subjects
Animals
Biomechanical Phenomena
Bone Regeneration
Cell Differentiation
Humans
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mice
Osteoblasts - cytology
Osteoblasts - metabolism
Osteogenesis
Stress, Mechanical
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:Scaffold architecture exerts a considerable influence on the osteogenic effect through stress transmission, as the deformation of scaffolds alters the mechanical microenvironment of cells adhering to scaffold surface. Despite extensive research on bone regeneration influenced by scaffold architecture, present studies have not addressed the biological mechanism underlying scaffold architecture-induced stress stimulation (SASS) on cells yet, posing a great challenge in revealing the biomechanical cues between scaffold architecture and osteogenic progression. Therefore, graphite, fullerene, and diamond scaffolds with gradient stress stimulation to cells after deformation were prepared. The cellular biomechanical mechanisms of SASS through single-cell RNA sequencing indicated that architectures providing SASS can induce the enrichment of focal adhesion and osteogenic differentiation pathways of bone mesenchymal stem cells and balance bone resorption of osteoclasts and bone formation of osteoblasts. Besides, SASS enhances bone regeneration for repairing critical-sized defects in vivo. These results provide insights for artificial bone scaffold design and clarify the biomechanical cues between SASS and osteogenic progression. Scaffold architecture stress mechanics stimulation mediates osteogenic progression in bone regeneration of artificial scaffolds.
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ISSN:2375-2548
2375-2548
DOI:10.1126/sciadv.adv8804