15-deoxy-Δ12,14-prostaglandin J2 reduces albumin-induced arthritis in temporomandibular joint of rats

• Published in: European journal of pharmacology Vol. 740; pp. 58 - 65
• Main Authors: Silva Quinteiro, Mariana, Henrique Napimoga, Marcelo, Gomes Macedo, Cristina, Furtado Freitas, Fabiana, Balassini Abdalla, Henrique, Bonfante, Ricardo, Trindade Clemente-Napimoga, Juliana
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.10.2014
• Subjects: 15d-PGJ2; Animals; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antigens; Arthritis, Experimental - drug therapy; Arthritis, Experimental - immunology; CD55 Antigens - immunology; Cell Movement - drug effects; Cytokines - immunology; Freund's Adjuvant; Inflammation; Injections, Intra-Articular; Intercellular Adhesion Molecule-1 - immunology; Leukocytes - drug effects; Leukocytes - physiology; Male; Prostaglandin D2 - analogs & derivatives; Prostaglandin D2 - pharmacology; Prostaglandin D2 - therapeutic use; Rats, Wistar; Serum Albumin, Bovine; Temporomandibular joint; Temporomandibular Joint Disorders - drug therapy; Temporomandibular Joint Disorders - immunology; 15d-PGJ2; Temporomandibular joint; Inflammation; 15d-PGJ
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2014.07.002

The aim of this study was to evaluate the peripheral effect of 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) in albumin-induced arthritis in temporomandibular joint (TMJ) of rats. Antigen-induced arthritis (AIA) was generated in rats with methylated bovine serum albumin (mBSA) diluted in complete Freund׳s adjuvant. Pretreatment with an intra-articular injection of 15d-PGJ2 (100ng/TMJ) before mBSA intra-articular injection (10µg/TMJ) (challenge) in immunized rats significantly reduced the albumin-induced arthritis inflammation. The results demonstrated that 15d-PGJ2 was able to inhibit plasma extravasation, leukocyte migration and the release of inflammatory cytokines IL-6, IL-12, IL-18 and the chemokine CINC-1 in the TMJ tissues. In addition, 15d-PGJ2 was able to increase the expression of the anti-adhesive molecule CD55 and the anti-inflammatory cytokine IL-10. Taken together, it is possible to suggest that 15d-PGJ2 inhibit leukocyte infiltration and subsequently inflammatory process, through a shift in the balance of the pro- and anti-adhesive properties. Thus, 15d-PGJ2 might be used as a potential anti-inflammatory drug to treat arthritis-induced inflammation of the temporomandibular joint.