Reduced signal crosstalk multi neurotransmitter image sensor by microhole array structure

A microhole array structure combined with an enzyme immobilization method using magnetic beads can enhance the target discernment capability of a multi neurotransmitter image sensor. Here we report the fabrication and evaluation of the H+-diffusion-preventing capability of the sensor with the array...

Full description

Saved in:
Bibliographic Details
Published inJapanese Journal of Applied Physics Vol. 57; no. 6S1; pp. 6 - 9
Main Authors Ogaeri, Yuta, Lee, You-Na, Mitsudome, Masato, Iwata, Tatsuya, Takahashi, Kazuhiro, Sawada, Kazuaki
Format Journal Article
LanguageEnglish
Published Tokyo The Japan Society of Applied Physics 01.06.2018
Japanese Journal of Applied Physics
Subjects
Beads
Biological properties
Crosstalk
Image enhancement
Microholes
Neurotransmitters
Photoresists
Pixels
Sensor arrays
Sensors
Online AccessGet full text

Cover

More Information
Summary:A microhole array structure combined with an enzyme immobilization method using magnetic beads can enhance the target discernment capability of a multi neurotransmitter image sensor. Here we report the fabrication and evaluation of the H+-diffusion-preventing capability of the sensor with the array structure. The structure with an SU-8 photoresist has holes with a size of 24.5 × 31.6 µm2. Sensors were prepared with the array structure of three different heights: 0, 15, and 60 µm. When the sensor has the structure of 60 µm height, 48% reduced output voltage is measured at a H+-sensitive null pixel that is located 75 µm from the acetylcholinesterase (AChE)-immobilized pixel, which is the starting point of H+ diffusion. The suppressed H+ immigration is shown in a two-dimensional (2D) image in real time. The sensor parameters, such as height of the array structure and measuring time, are optimized experimentally. The sensor is expected to effectively distinguish various neurotransmitters in biological samples.
ISSN:0021-4922
1347-4065
DOI:10.7567/JJAP.57.06HH01