OTULIN haploinsufficiency predisposes to environmentally directed inflammation

Recently, OTULIN haploinsufficiency was linked to enhanced susceptibility to infections accompanied by local necrosis and systemic inflammation. The pathogenesis observed in haploinsufficient patients differs from the hyperinflammation seen in classical OTULIN-related autoinflammatory syndrome (ORAS...

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Published inFrontiers in immunology Vol. 15; p. 983686
Main Authors Staels, Frederik, Bücken, Leoni, De Vuyst, Leana, Willemsen, Mathijs, Van Nieuwenhove, Erika, Gerbaux, Margaux, Neumann, Julika, Malviya, Vanshika, Van Meerbeeck, Lize, Haughton, Jeason, Seldeslachts, Laura, Gouwy, Mieke, Martinod, Kimberly, Vande Velde, Greetje, Proost, Paul, Yshii, Lidia, Schlenner, Susan, Schrijvers, Rik, Liston, Adrian, Humblet-Baron, Stephanie
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 21.05.2024
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Summary:Recently, OTULIN haploinsufficiency was linked to enhanced susceptibility to infections accompanied by local necrosis and systemic inflammation. The pathogenesis observed in haploinsufficient patients differs from the hyperinflammation seen in classical OTULIN-related autoinflammatory syndrome (ORAS) patients and is characterized by increased susceptibility of dermal fibroblasts to alpha toxin-inflicted cytotoxic damage. Immunological abnormalities were not observed in OTULIN haploinsufficient patients, suggesting a non-hematopoietic basis. In this research report, we investigated an mouse model after provocation with lipopolysaccharide (LPS) to explore the potential role of hematopoietic-driven inflammation in OTULIN haploinsufficiency. We observed a hyperinflammatory signature in LPS-provoked mice, which was driven by CD64 monocytes and macrophages. Bone marrow-derived macrophages (BMDMs) of mice demonstrated higher proinflammatory cytokine secretion after stimulation with LPS or polyinosinic:polycytidylic acid (Poly(I:C)). Our experiments in full and mixed bone marrow chimeric mice suggest that, in contrast to humans, the observed inflammation was mainly driven by the hematopoietic compartment with cell-extrinsic effects likely contributing to inflammatory outcomes. Using an OTULIN haploinsufficient mouse model, we validated the role of OTULIN in the regulation of environmentally directed inflammation.
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ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2024.983686