Virus transmission through interspecies protoplast fusion in Aspergillus

Protoplast fusion between the virus-infected species Aspergillus niger, a glucoamylase producer, and the virus-free species A. oryzae and A. ficuum was carried out. The presence or absence of polyhedral virus particles, of 28–33 nm diam, in their hybrids and segregants of both hybrids and heterokary...

Full description

Saved in:
Bibliographic Details
Published inTransactions of the British Mycological Society Vol. 89; no. 1; pp. 73 - 81
Main Authors Pingyan, Liang, Kaiying, Chen
Format Journal Article
LanguageEnglish
Published London Cambridge University Press 01.07.1987
Subjects
Aspergillus
Aspergillus ficuum
Aspergillus niger
Aspergillus oryzae
Biological and medical sciences
disease transmission
Fundamental and applied biological sciences. Psychology
Microbiology
protoplast fusion
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Virology
viruses
Fungi
Virus
Aspergillus
Protoplast
Transmission
Fungi Imperfecti
Thallophyta
Online AccessGet full text

Cover

More Information
Summary:Protoplast fusion between the virus-infected species Aspergillus niger, a glucoamylase producer, and the virus-free species A. oryzae and A. ficuum was carried out. The presence or absence of polyhedral virus particles, of 28–33 nm diam, in their hybrids and segregants of both hybrids and heterokaryons were detected by means of electron microscopy, immune double-diffusion assay and gel electrophoresis. Virus particles were present in hybrids of both combinations and in most of the segregants, except those which resembled the original virus-free phenotype. Comparison of glucoamylase production by parental strains, their hybrids and segregants indicated that the hybrid expressed the advantage of high yield of enzyme and growth rate.
ISSN:0007-1536
DOI:10.1016/S0007-1536(87)80060-8