Effects of replacing dietary fish meal with Clostridium autoethanogenum protein on growth and flesh quality of Pacific white shrimp (Litopenaeus vannamei)

• Published in: Aquaculture Vol. 549; p. 737770
• Main Authors: Yao, Wenxiang, Yang, Pinxian, Zhang, Xin, Xu, Xiaoying, Zhang, Chunyan, Li, Xiaoqin, Leng, Xiangjun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.02.2022
• Subjects: Clostridium autoethanogenum protein; Flesh quality; Growth; Litopenaeus vannamei; Metabolomics; Metabolomics; Flesh quality; Litopenaeus vannamei; Clostridium autoethanogenum protein; Growth
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2021.737770

This study evaluated the effects of replacing fish meal (FM) with Clostridium autoethanogenum protein (CAP) on growth and flesh quality of Pacific white shrimp, Litopenaeus vannamei. A control diet was designed to contain 560 g/kg FM, then 0%, 15%, 30%, 45%, 70% and 100% of dietary FM was replaced by CAP, referring to Con, CAP-15, CAP-30, CAP-45, CAP-70 and CAP-100, respectively. The six isoproteic and isolipidic diets were fed to shrimp (2.78 g) for 8 weeks. The increasing dietary CAP level negatively affected feed intake, weight gain and feed conversion ratio (P < 0.05). A negative linear tend was found between dietary CAP level and body surface redness, yellowness and flesh total collagen contents, n-3/n-6 PUFAs value and free flavor amino acids contents (P < 0.05). Both linear and quadratic trends were detected between flesh hardness, shear force, steaming loss, cooking loss and dietary CAP level (P < 0.05). In CAP-15 and CAP-30 groups, the above indexes presented the similar levels to the Con group (P > 0.05). Metabonomics analysis showed that the high replacement of FM with CAP affected the amino acids and fatty acids metabolism in flesh. In conclusion, in a basal diet containing 560 g/kg FM, 30% dietary FM could be successfully replaced by CAP, while higher FM substitution decreased the growth and flesh quality of L. vannamei. •CAP could replace 30% of dietary fish meal without significant effects on growth and flesh quality of Pacific white shrimp.•High inclusion of CAP (≥45%) decreased the growth and flesh quality.•High replacement of fish meal with CAP affected the lipid and amino acids metabolism.