Targeting Collagen Type III in Proteinuric Kidney Disease: Informing Drug Potential Using the Jaccard–Tanimoto Index

Collagenofibrotic glomerulopathy, a collagen type III kidney disease, is associated with proteinuria and accumulation ofcollagen type III in the glomerulus specifically the mesangium and/or capillary walls. The puromcyin aminonucleoside (PAN) nephropathy model was evaluated to examine the relation b...

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Published inProcesses Vol. 8; no. 8; p. 996
Main Authors Liu, Michelle, Dalvi, Anoushka, Dalapati, Sony, Prakash, Natalia, Hu, Zhijian, Zhou, Ping, Jiang, Kai, Pellicano, Anthony, Goldberg, Itzhak D., Narayan, Prakash
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2020
Subjects
Animal models
Collagen
Collagen (type III)
Diabetes
Glomerulus
Hypertrophy
Kidney diseases
Localization
Mesangium
Microscopy
Nephropathy
Ontology
Proteins
Proteinuria
Robustness
Urine
United States > US
Germany
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Summary:Collagenofibrotic glomerulopathy, a collagen type III kidney disease, is associated with proteinuria and accumulation ofcollagen type III in the glomerulus specifically the mesangium and/or capillary walls. The puromcyin aminonucleoside (PAN) nephropathy model was evaluated to examine the relation between COL3A1 mRNA and proteinuria. In Wistar rats administered PAN, a robust increase in proteinuria was accompanied by glomerular hypertrophy and expansion of both the Bowman’s capsule and Bowman’s space. An ~4-fold increase in renal COL3A1 mRNA was observed in the PAN cohort with urine protein exhibiting a direct (r = 0.8) and significant correlation with kidney COL3A1 mRNA level. Both Picrosirius red polarized microscopy and immunohistochemical analysis showed localization of collagen type III to the glomerular mesangium. Gene ontology-driven transcriptomic analysis reveals a robust COL3A1 network in the glomerular compartment.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr8080996