Humoral hypercalcaemia of malignancy: report of two further patients with biochemical studies on tumour extracts

• Published in: Clinical science (1979) Vol. 71; no. 3; pp. 261 - 269
• Main Authors: SESHADRI, M. S, MASON, R. S, CORNISH, C. J, PRYKE, A. M, POSEN, S
• Format: Journal Article
• Language: English
• Published: London Portland Press 01.09.1986
• Subjects: Adenylyl Cyclases - biosynthesis; Adult; Aged; Biological and medical sciences; Carcinoma, Renal Cell - analysis; Carcinoma, Renal Cell - complications; Chromatography, High Pressure Liquid; Cyclic AMP - biosynthesis; Female; Host-tumor relations. Immunology. Biological markers; Humans; Hypercalcemia - etiology; Hypercalcemia - metabolism; Kidney - metabolism; Kidney Neoplasms - analysis; Kidney Neoplasms - complications; Leg; Male; Medical sciences; Melanoma - analysis; Melanoma - complications; Osteosarcoma - metabolism; Parathyroid Hormone - analysis; Parathyroid Hormone - pharmacology; Tumors; Human; Hypercalcemia; Adenylate cyclase; Parathyroid hormone; Pathogenesis; Tumoral tissue; Stimulation; Tumor
• ISSN: 0143-5221; 1470-8736
• DOI: 10.1042/cs0710261

Tumour extracts from two patients with humoral hypercalcaemia of malignancy contained material which stimulated adenylate cyclase in chick renal membranes and in rat osteosarcoma cells. Adenylate cyclase-stimulating activity in each system was inhibited by a specific parathyroid hormone (PTH) antagonist. Studies in two HPLC systems suggested that the adenylate cyclase-stimulating factors extracted from these tumours differed from each other and from synthetic human parathyroid hormone 1-34. The presence of similar PTH-like adenylate cyclase stimulating material(s) in oncogenic osteomalacia suggests that adenylate cyclase stimulating factor(s) may not be the direct or the sole cause of hypercalcaemia.