Effect of maternal true combined endodontic-periodontal lesion on insulin and inflammatory pathway in adult offspring

• Published in: Journal of periodontology (1970) Vol. 94; no. 4; p. 487
• Main Authors: Belardi, Bianca Elvira, Mattera, Maria Sara de Lima Coutinho, Tsosura, Thaís Verônica Saori, Dos Santos, Rodrigo Martins, Cantiga-Silva, Cristiane, Tavares Ângelo Cintra, Luciano, Chiba, Fernando Yamamoto, Bravo, Lara Teschi, Carvalho, Nubia Ramos, Carnevali, Ana Carolina Nascimento, de Oliveira, Renan Akira Fujii, Ervolino, Edilson, Matsushita, Doris Hissako
• Format: Journal Article
• Language: English
• Published: United States 01.04.2023
• Subjects: Animals; Female; I-kappa B Kinase - metabolism; Inflammation; Insulin; Insulin Resistance - physiology; JNK Mitogen-Activated Protein Kinases - metabolism; Periodontal Diseases; Rats; Rats, Wistar; Tumor Necrosis Factor-alpha - metabolism; tumor necrosis factor-alpha; low birth weight; periodontal diseases; apical periodontitis; insulin resistance
• ISSN: 1943-3670
• DOI: 10.1002/JPER.22-0139

Maternal periodontal disease (PED) and apical periodontitis (AP) are associated insulin resistance (IR), increased tumor necrosis factor-α (TNF-α) levels, and alterations in insulin signaling (IS) in the gastrocnemius muscle (GM) of adult offspring. TNF-α stimulates I kappa B kinase (IKK) and c-Jun N-terminal protein kinase (JNK), resulting in IS attenuation. However, studies that investigated the maternal true endodontic-periodontal lesion (EPL) in offspring are scarce, and in this case, the impact could be even higher. This study aimed to evaluate the effects of EPL on the IR, IS, and inflammatory pathways on the offspring GM. Female Wistar rats were distributed into control, AP, PED, and EPL groups. After 30 days of oral inflammation induction, rats from all groups were allowed to mate with healthy rats. The body weight of the offspring was assessed from birth to 75 days of age. After 75 days, the following measurements were performed: glycemia, insulinemia, IR, TNF-α content, and IKKα/β, JNK, pp185 (Tyr), and IRS-1 (Ser) phosphorylation status in the GM. Maternal PED and EPL were associated with low birth weights. All maternal oral inflammations promoted IR and IS impairment in the GM and only maternal PED and EPL caused an increase in TNF-α content and IKKα/β phosphorylation status in the GM of offspring. The offspring of the rats with EPL group showed worsening of metabolic changes when compared with offspring of rats with AP or PED. Association of maternal AP and PED promoted a more pronounced worsening in the health of the adult offspring.