The Effect of Urocortin 1 on Motility in Isolated, Vascularly Perfused Rat Colon

• Published in: The Korean journal of gastroenterology Vol. 65; no. 5; pp. 283 - 290
• Main Authors: You, Il Young, Lee, Seungho, Kim, Ki Bae, Lee, Hee Seung, Jang, Jong Soon, Yeon, Myeongho, Han, Joung-Ho, Yoon, Soon Man, Chae, Hee Bok, Park, Seon Mee, Youn, Sei Jin
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한소화기학회 01.05.2015
• Subjects: Animals; Colon - drug effects; Colon - physiology; Injections, Intravenous; Male; Muscle Contraction - drug effects; Neurotransmitter Agents - pharmacology; Rats; Rats, Sprague-Dawley; Receptors, Cholinergic - chemistry; Receptors, Cholinergic - metabolism; Urocortins - isolation & purification; Urocortins - pharmacology; 내과학; Urocortins; Rats; Corticotropin-releasing factor; Cholinergic receptors; Muscarinic receptors
• ISSN: 1598-9992; 2233-6869
• DOI: 10.4166/kjg.2015.65.5.283

Urocortin 1, a corticotropin-releasing factor related peptide, increases colonic motility under stressful conditions. We investigated the effect of urocortin 1 on colonic motility using an experimental model with isolated rat colon in which the blood flow and intestinal nerves were preserved. Furthermore, we assessed whether this effect was mediated by adrenergic or cholinergic nerves. Colonic motility was measured in the proximal and distal parts of resected rat colon. The colon resected from the peritoneum was stabilized, and then urocortin 1 (13.8, 138, 277, and 1,388 pM) was administered via a blood vessel. Motility index was measured in the last 5 min of the 15 min administration of urocortin 1 and expressed as percentage change from baseline. Subsequently, the change in motility was measured by perfusing urocortin 1 in colons pretreated with phentolamine, propranolol, hexamethonium, atropine, or tetrodotoxin. At concentrations of 13.8, 138, 277, and 1,388 pM, urocortin 1 increased the motility of proximal colon (20.4 ± 7.2%,48.4 ± 20.9%, 67.0 ± 25.8%, and 64.2 ± 20.9%, respectively) and the motility of distal colon (3.3 ± 3.3%, 7.8 ± 7.8%, 71.1 ± 28.6%,and 87.4 ± 32.5%, respectively). The motility induced by urocortin 1 was significantly decreased by atropine to 2.4 ± 2.4% in proximal colon and 3.4 ± 3.4% in distal colon (p <œ 0.05). However, tetrodotoxin, propranolol, phentolamine, and hexamethonium did not inhibit motility. Urocortin 1 increased colonic motility and it is considered that this effect was directly mediated by local muscarinic cholinergic receptors.