Differentiation of Black Raspberry Fruits According to Species and Geographic Origins by Genomic Analysis and ¹H-NMR-based Metabolic Profiling

• Published in: Applied biological chemistry Vol. 55; no. 5; pp. 633 - 642
• Main Authors: Park, S.J., Chung-Ang University, Seoul, Republic of Korea, Hyun, S.H., Chung-Ang University, Seoul, Republic of Korea, Suh, H.W., Chung-Ang University, Seoul, Republic of Korea, Lee, S.Y., Chung-Ang University, Seoul, Republic of Korea, Min, T.S., National Research Foundation of Korea, Daejeon, Republic of Korea, Auh, J.H., Chung-Ang University, Ansung, Republic of Korea, Lee, H.J., Chung-Ang University, Ansung, Republic of Korea, Kim, J.H., Chung-Ang University, Seoul, Republic of Korea, Cho, S.M., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Choi, H.K., Chung-Ang University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.10.2012; Springer Nature B.V; 한국응용생명화학회
• Subjects: Amino acids; Anthocyanins; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; black raspberry; Chemistry; Chemistry and Materials Science; data collection; Deoxyribonucleic acid; DNA; DNA fingerprinting; Flavanols; Flavonoids; fruit extracts; Fruits; Genetic analysis; Genetic fingerprinting; Genomic analysis; genomics; geographic origin; Kerria japonica; Leaves; Metabolism; metabolomics; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Organic acids; Original Article; Origins; Phenolic acids; Phenols; provenance; Random amplified polymorphic DNA; random amplified polymorphic DNA technique; RAPD; Rubus coreanus; Rubus occidentalis; Species; Sugar; sugars; 농학; geographic origin; random amplification of polymorphic DNA; black raspberry; H-NMR; metabolomics
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.1007/s13765-012-2062-0

Random amplification of polymorphic DNA (RAPD) and NMR techniques were used to differentiate and identify species of black raspberry (BR) of different geographic origins. BR leaf samples from five geographic origins were identified as Rubus japonicus and R. coreanus by RAPD. ¹H-NMR analysis was also performed for BR fruit extracts from different geographic origins. Major compounds assigned in ¹H-NMR spectra of BR fruits were amino acids, organic acids, sugars, phenolic acids, and purine derivatives. In addition, relative levels of total phenolic compounds, flavanols, flavonoids, and anthocyanins in the BR fruits were further analyzed. Hierarchical cluster analyses (HCA) based on the genetic (RAPD of leaf samples) and metabolic (¹H-NMR, total phenolic compounds, flavanols, flavonoids and anthocyanins of fruit samples) datasets were independently performed. The HCA dendrogram pattern derived from RAPD genetic fingerprinting of BR leaf samples matched that from the ¹H-NMR data of BR fruit samples. This research demonstrates that metabolic profiling of BR fruit based on ¹H-NMR is a promising method for differentiating BR fruits of various species and geographic origins.