Protease-catalysed peptide synthesis in solvent-free system

A new method of enzymatic peptide synthesis without any liquid added has been proposed. The method is based on the admixing of N-acylamino acid (peptide) esters and nucleophiles (amides or tert.-butylesters of amino acids or peptides, peptides) with various proteolytic enzymes such as alpha -chymotr...

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Bibliographic Details
Published inBiotechnology techniques Vol. 6; no. 2; pp. 155 - 160
Main Author Cerovsky, V
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 01.03.1992
Subjects
Biological and medical sciences
Biotechnology
enzymatic syntheses
Enzyme engineering
esters
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
nucleophiles
peptide synthesis
proteolytic enzymes
Enzymatic catalysis
Peptide synthesis
Method
Enzyme
Proteinase
Operating conditions
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Summary:A new method of enzymatic peptide synthesis without any liquid added has been proposed. The method is based on the admixing of N-acylamino acid (peptide) esters and nucleophiles (amides or tert.-butylesters of amino acids or peptides, peptides) with various proteolytic enzymes such as alpha -chymotrypsin, trypsin, proteinase K, subtilisin, elastase and papain in the presence of Na sub(2)CO sub(3).10H sub(2)O. In most instances, acylating components were completely converted within a few hours giving satisfactory yields of desired peptide products.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1007/BF02438823