Development of a Spectrophotometric Method for the Determination of Florfenicol in Eudragit Nanocapsules

The objective of this work was to develop a simple spectrophotometric method for determining antibiotic florfenicol in Eudragit nanocapsules. Regression curve of the method is linear in a concentration range of 80 – 480 μg/mL (correlation coefficient, 0.9998) at a selected wavelength of 270 nm. The...

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Bibliographic Details
Published inPharmaceutical chemistry journal Vol. 54; no. 11; pp. 1181 - 1185
Main Authors Castañeda, Pablo Serrano, Olvera, Lilia Gutiérrez, Bernad, María Josefa Bernad, López, Héctor Sumano, Escobar-Chávez, José Juan
Format Journal Article
LanguageEnglish
Published New York Springer US 01.02.2021
Subjects
Medicine
Organic Chemistry
Pharmacology/Toxicology
Pharmacy
florfenicol
spectrophotometric determination
Eudragit nanocapsules
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Summary:The objective of this work was to develop a simple spectrophotometric method for determining antibiotic florfenicol in Eudragit nanocapsules. Regression curve of the method is linear in a concentration range of 80 – 480 μg/mL (correlation coefficient, 0.9998) at a selected wavelength of 270 nm. The limits of detection and quantification are 0.4 μg/mL and 1.2 μg/mL, respectively. The developed method demonstrated good inter-day and intra-day precision with RSD < 2. The percentage recovery of florfenicol was within 99.56 – 99.90%. In conclusion, the proposed method is specific, reproducible, accurate, and robust for the determination of florfenicol in Eudragit nanocapsules with detection at 270 nm in 95% confidence interval.
ISSN:0091-150X
1573-9031
DOI:10.1007/s11094-021-02340-0